HiScreen Capto adhere ImpRes

• Cat#No#: Hi-286P
• Product Overview: HiScreen Capto adhere ImpRes is a ready-to-use column pre-packed with BioProcess Capto adhere ImpRes strong anion exchange multimodal resin. The columns are an excellent choice for method optimization and parameter screening.
• Description: The multimodal functionality of Capto adhere ImpRes gives a different selectivity compared with traditional ion exchange columns and it binds proteins at high or low ionic strength.
• Characteristic: High yields achieved through the high-resolution beads and selectivity of the ligand.
  Efficient removal of aggregates, viruses, and main contaminants in MAb processes.
  10 cm bed height of HiScreen columns allows method optimization and parameter screening.
  Reproducible results, scalable to BioProcess columns packed with the same chromatography resins using the same linear fluid velocity.
• Sample preparation: 1. Adjust the sample to the composition of the start buffer, using one of these methods: Dilute the sample with start buffer. Exchange buffer using a HiPrep 26/10 Desalting, HiTrap Desalting or PD-10 Desalting column.
  2. Filter the sample through a 0.45 µm filter or centrifuge at 10 000 × g for 10 min immediately before loading it to the column. This prevents clogging and increases the life time of the column when loading large sample volumes.
• Metal ion capacity: 0.08 to 0.11 mmol Cl- /mL medium
• Matrix: Highly cross-linked agarose
• Particle Size: 36 to 44 µm
• Recommended flow rate: 100 to 300 cm/h
• Chemical stability: All commonly used aqueous buffers, 1 M acetic acid, 1 M sodium hydroxide.
• pH working range: 3 to 12
• CIP stability: 2 to 14
• Storage: 4°C to 30°C in 20% ethanol
• Cleaning-in-place: 1. Wash with at least 2 column volumes (CV) of 2 M NaCl.
  2. Wash with at least 3 CV 1 M NaOH with at least 15 min contact time.
  3. Wash with at least 2 CV 2 M NaCl.
  4. Wash with at least 2 CV distilled water.
  5. Wash with 5 CV start buffer or until eluent pH and conductivity have reached the required values.
• Pack size: 1 × 4.7 mL
• Maximum flow velocity: 300 cm/h
• Column hardware pressure limit: 8 bar (0.8 MPa)
• Functional group: multimodal strong anion exchanger