HiScreen Capto adhere

• Cat#No#: Hi-219P
• Product Overview: HiScreen Capto adhere columns are prepacked with Capto adhere ion exchange chromatography resins. The columns are an excellent choice for method optimization and parameter screening.
• Description: The small column volume of 4.7 mL and bed height of 10 cm make HiScreen columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up.
• Characteristic: Capto adhere is a multimodal anion exchanger for intermediate purification and polishing of monoclonal antibodies after the protein A capture step, giving the possibility to design a two-step chromatographic downstream process.
  Prepacked, ready-to-use columns for convenient process development optimization.
  10 cm bed height of HiScreen columns is designed to allow method optimization and parameter screening.
  Easily connected in series to achieve 20 cm bed height.
  Small bed volume gives fast results and minimal sample/buffer consumption.
  Reproducible results, scalable to BioProcess columns packed with the same resin using the same linear fluid velocity.
• Sample preparation: 1. Adjust the sample to the composition of the start buffer, using one of these methods: Dilute the sample with start buffer. Exchange buffer using a HiPrep 26/10 Desalting, HiTrap Desalting or PD-10 Desalting column.
  2. Filter the sample through a 0.45 µm filter or centrifuge at 10 000 × g for 10 min immediately before loading it to the column. This prevents clogging and increases the life time of the column when loading large sample volumes.
• Metal ion capacity: 0.09 to 0.12 mmol Cl- /mL resin
• Matrix: Highly cross-linked agarose, spherical
• Ionic Exchanger Type: Multimodal strong anion exchanger
• Average particle size: ~ 75 µm
• Recommended flow rate: < 600 cm/h
• Recommended column height: 100 mm
• Chemical stability: Stable to commonly used aqueous buffers, 1 M acetic acid, 1.0 M NaOH.
• pH working range: 3 to 12
• CIP stability: 2 to 14
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: 1. Wash with at least 2 column volumes (CV) of 2.0 M NaCl.
  2. Wash with at least 4 CV 1.0 M NaOH.
  3. Wash with at least 2 CV 2.0 M NaCl.
  4. Wash with at least 2 CV ultra pure water.
  5. Wash with Capto DEAE At least 10 CV start buffer or until eluent pH and conductivity have reached the required values.
• Pack size: 1 × 4.7 mL
• Dimensions: 7.7 × 100 mm
• Column volume: 4.7 mL
• Column i.d.: 7.7 mm
• Column hardware pressure limit: 0.8 MPa (8 bar, 115 psi)