HiScreen Capto Blue

• Cat#No#: Hi-065P
• Maximum operating pressure: 3 bar [0.3 MPa] (44 psi)
• Matrix: Cross-linked agarose, 6%, spherical
• Average particle size: ~ 75 µm
• Ligand density: 11 to 16 µmol/ml Cibacron Blue/mL resin
• Dynamic binding capacity: Approx. 25 mg human serum albumin/ml medium
• Recommended flow rate: 1.2 ml/min - 2.3 ml/min
• Recommended column height: 100 mm
• Chemical stability: Stable to commonly used aqueous buffers, 0.01 M NaOH 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol.
• pH working range: 3 to 13
• CIP stability: 2 to 13.5
• Storage: 2°C to 8°C
• Cleaning-in-place: Precipitated proteins: 1. Wash the column with 4 column volumes (CV) of either 0.5 M (HiScreen Capto Blue) or 0.1 M NaOH (HiScreen Blue FF) at 40 cm/h. 2. Wash with 3 to 4 CV of 70% ethanol or 2 M potassium thiocyanate. 3. Wash immediately with at least 5 CV filtered start buffer, pH 8.0. or 1. Wash the column with 2 CV of 6 M guanidine hydrochloride. 2. Wash immediately with at least 5 CV filtered start buffer, pH 8.0.
  Strongly bound hydrophobic proteins, lipoproteins, and lipids:1. Wash the column with 3 to 4 CV of up to 70% ethanol or 30% isopropanol.. 2. Wash immediately with at least 5 CV filtered start buffer, pH 8.0. or 1. Wash with 2 CV detergent in a basic or acidic solution, e.g., 0.1% non-ionic detergent in 1 M acetic acid. Wash at a flow rate of 40 cm/h. 2. Remove residual detergent by washing with 5 CV of 70% ethanol. 3. Wash immediately with at least 5 CV filtered start buffer, pH 8.0.
• Maximum flow velocity: 4.7 mL/min
• Dimensions: 7.7 × 100 mm
• Column volume: 4.7 ml
• Column i.d.: 7.7 mm
• Column hardware pressure limit: 0.8 MPa (8 bar, 116 psi)