HiScreen Capto Butyl ImpRes

• Cat#No#: Hi-139P
• Product Overview: HiScreen Capto Butyl ImpRes is a ready-to-use column prepacked with Capto Butyl ImpRes hydrophobic interaction chromatography resin and is part of Cytiva's process development platform.
• Description: Capto Butyl ImpRes is hydrophobic interaction chromatography (HIC) media (resins) developed for the intermediate and polishing steps in a downstream protein purification process. Both chromatography media extend the well established Capto platform to include high-resolution media. By combining the high-flow characteristics of Capto media with a smaller particle size, Capto Phenyl ImpRes and Capto Butyl ImpRes deliver both excellent pressure/flow properties and resolution. The ability to run at higher flow velocities and higher bed heights increases flexib
• Characteristic: Excellent choice for method optimization and parameter screening with a 10 cm bed height.
  Easily connected in series to achieve 20 cm bed height.
  Small bed volume gives fast results and minimal sample/buffer consumption.
  Reproducible results, scalable to BioProcess columns packed with the same chromatography resin using the same linear fluid velocity.
• Maximum operating pressure: 3 bar [0.3 MPa] (44 psi)
• Matrix: High-flow agarose
• Average particle size: 40 μm
• Ligand: Butyl
• Dynamic binding capacity: 37 mg BSA/mL medium
• Recommended flow rate: Up to 220 cm/h in a 1 m diameter column with a bed height of 20 cm at 20°C; measured using process buffers with the same viscosity as water at 300 kPa.
• Recommended column height: 100 mm
• Chemical stability: Stable in commonly used aqueous buffers: 1 M sodium hydroxide††, 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 70% ethanol, 30% isopropanol.
• pH working range: 3 to 13
• CIP stability: 2 to 14
• Shelf life: Five years
• Storage: 20% ethanol at 4°C to 30°C
• Cleaning-in-place: 1 M NaOH
• Pack size: 4.7 mL
• Dimensions: 7.7 × 100 mm
• Column i.d.: 7.7 mm
• Column hardware pressure limit: 0.8 MPa (8 bar, 116 psi)