HiScreen Capto Core 700

• Cat#No#: Hi-284P
• Product Overview: Capto Core 700 is designed for intermediate purification and polishing of viruses and other large biomolecules. The novel core bead technology and multimodal, octylamine ligand give Capto Core 700 dual functionality, size exclusion, and binding chromatography in one chromatography resin.
• Description: Capto Core 700 is composed of a ligand-activated core and inactive shell. The inactive shell excludes large molecules (cut off ~ Mr 700 000 [700 kDa]) from entering the core through the pores of the shell. These larger molecules are collected in the column flowthrough while smaller impurities bind to the internalized ligands. The core of each bead is functionalized with ligands that are both hydrophobic and positively charged, resulting in a highly efficient multimodal binding of various contaminants small enough to enter the core. The multimodal ligands ensure strong binding with most impurities over a wide range of pH and salt concentrations. Bound impurities are removed from the beads by cleaning-in-place (CIP) procedures using NaOH and in most cases, a solvent.
• Characteristic: For intermediate purification and polishing of viruses and other large biomolecules (Mr > 350 000) in flowthrough mode.
  Novel core bead technology allows efficient capture of contaminants, while target molecule is collected in the flowthrough. Significantly improved productivity and higher flow rates compared with gel filtration methods.
  Straightforward optimization due to flowthrough chromatography and robust performance.
  Convenient small-scale purification, process development, and scale-up using prepacked HiTrap and HiScreen columns.
• Maximum operating pressure: 8 bar [0.8 MPa]
• Matrix: Highly cross‑linked agarose
• Average particle size: 85 μm
• Dynamic binding capacity: 13 mg ovalbumin/mL resin
• Recommended flow rate: 0.8-3.9 ml/min
• Recommended column height: 100 mm
• Chemical stability: All commonly used aqueous buffers, 1 M sodium hydroxide (NaOH), 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol.
• pH working range: 3 to 13
• CIP stability: 3 to 14
• Storage: 20% ethanol at 4°C to 30°C
• Cleaning-in-place: Use of 1 M NaOH in 27% 1‑propanol is recommended for effective CIP and sanitization of the resin after every cycle.
• Pack size: 1 × 4.7 mL
• Maximum flow velocity: 500 cm/h in column with 20 cm bed height at < 2 bar (0.2 MPa).
• Dimensions: 7.7 × 100 mm
• Column volume: 4.7 mL
• Column i.d.: 7.7 mm
• Column hardware pressure limit: 0.8 MPa (8 bar, 116 psi)