HiScreen Capto L

• Cat#No#: Hi-006P
• Product Overview: HiScreen Capto L columns are prepacked with Capto L, an affinity chromatography resin for capture of antibodies and antibody fragments such as Fab fragments, scFv, and Dabs. HiScreen Capto L columns are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.
• Description: HiScreen columns are prepacked with a range of BioProcess chromatography resins and are designed for method optimization and parameter screening. HiScreen columns have small bed volumes (4.7 mL), reducing the cost of sample and buffer consumption. The resins used in HiScreen columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.
• Characteristic: Prepacked, ready-to-use columns for convenient process development optimization.
  10 cm bed height of HiScreen columns is designed to allow method optimization and parameter screening.
  Easily connected in series to achieve 20 cm bed height.
  Small bed volume (4.7 mL) gives fast results and minimal sample/buffer consumption.
  Reproducible results, scalable to BioProcess columns packed with the same resin using the same linear fluid velocity.
• Maximum operating pressure: 3 bar [0.3 MPa] (44 psi)
• Average particle size: 85 µm
• Ligand: Recombinant protein L (E. coli)
• Coupling chemistry: Epoxy
• Dynamic binding capacity: ~ 25 mg Fab
• Recommended flow rate: 100 to 300 cm/h
• Recommended column height: 100 mm
• Chemical stability: All commonly used aqueous buffers, e.g., 15 mM sodium hydroxide, 8 M urea, 6 M guanidine hydrochloride, 1 M acetic acid.
• pH working range: 2 to 10
• Temperature stability: 2°C to 40°C
• Storage: 2 to 8°C, 20% Ethanol
• Cleaning-in-place: 1. Wash the column with 3 CV binding buffer.
  2. Wash with at least 2 CV 15 mM NaOH. Contact time 10 to 15 minutes.
  3. Wash immediately with at least 5 CV binding buffer.
• Pack size: 1 × 4.7 mL
• Maximum flow velocity: 500 cm/h
• Dimensions: 7.7 × 100 mm
• Column volume: 4.7 ml
• Column i.d.: 7.7 mm
• Column hardware pressure limit: 8 bar (0.8 MPa, 117 psi)