HiScreen Capto MMC ImpRes

• Cat#No#: Hi-285P
• Product Overview: HiScreen Capto MMC ImpRes is a ready-to-use column pre-packed with BioProcess Capto MMC ImpRes weak cation exchange multimodal resin. The columns are an excellent choice for method optimization and parameter screening.
• Description: The multimodal functionality of Capto MMC ImpRes gives a different selectivity compared with traditional ion exchange columns and it binds proteins at high or low ionic strength.
• Characteristic: High yields achieved through the high-resolution beads and selectivity of the ligand.
  Enables use of a platform approach to MAb process development.
  Displays a broad pH/salt operational window.
  10 cm bed height of HiScreen columns allows method optimization and parameter screening.
  Reproducible results, scalable to BioProcess columns packed with the same chromatography resin using the same linear fluid velocity.
• Maximum operating pressure: 0.3 MPa (3 bar, 43.5 psi) at min. 220 cm/h, 1 m diameter column, 20 cm bed height
• Sample preparation: 1. Adjust the sample to the composition of the start buffer, using one of these methods: Dilute the sample with start buffer. Exchange buffer using a HiPrep 26/10 Desalting, HiTrap Desalting or PD-10 Desalting column.
  2. Filter the sample through a 0.45 µm filter or centrifuge at 10 000 × g for 10 min immediately before loading it to the column. This prevents clogging and increases the life time of the column when loading large sample volumes.
• Metal ion capacity: 25 to 39 µmol/mL
• Matrix: High-flow agarose
• Particle Size: 36 to 44 μm
• Ligand: Multimodal weak cation exchanger
• Ligand density: 25 to 39 µmol ligand/ml medium
• Dynamic binding capacity: 60 to 90 mg MAb/mL (residence time 4 to 5 min)
• Chemical stability: All commonly used aqueous buffers, 1 M NaOH, 2 M NaCl, 5% 1-propanol, 30% isopropanol, 70% ethanol.
• pH working range: 3 to 12
• CIP stability: 2 to 14
• Storage: 4°C to 30°C in 20% ethanol, 0.2 M sodium acetate.
• Cleaning-in-place: 1. Wash with at least 2 column volumes (CV) of 2 M NaCl.
  2. Wash with at least 3 CV 1 M NaOH with at least 15 min contact time.
  3. Wash with at least 2 CV 2 M NaCl.
  4. Wash with at least 2 CV distilled water.
  5. Wash with 5 CV start buffer or until eluent pH and conductivity have reached the required values.
• Pack size: 1 × 4.7 mL
• Maximum flow velocity: 300 cm/h
• Column hardware pressure limit: 8 bar (0.8 MPa)