HiScreen Capto Phenyl (High Sub)

• Cat#No#: Hi-140P
• Product Overview: Capto Phenyl (High sub) is hydrophobic interaction chromatography (HIC) resins used in the capture and intermediate stages of protein purification.
• Description: HiScreen columns are prepacked with a range of BioProcess chromatography resins (resins) and designed for method optimization and parameter screening. HiScreen columns have small bed volumes (4.7 mL), reducing the cost of sample and buffer consumption. The resins used in HiScreen columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.
• Characteristic: Prepacked with Capto Phenyl (high sub).
  Excellent for method optimization and parameter screening due to the 10 cm bed height.
  Easily connected in series to achieve 20 cm bed height.
  Small bed volume for fast results and minimal sample/buffer consumption.
  Reproducible results, scalable to BioProcess columns packed with the same resin using the same flow velocity.
• Maximum operating pressure: At least 600 cm/h at < 0.3 MPa in a 1 m diameter column and 20 cm bed height (at 20°C using process buffers with the same viscosity as water)
• Matrix: Highly cross-linked agarose, spherical
• Average particle size: ~ 75 µm
• Ligand: Phenyl
• Ligand density: ~ 27 µmol Phenyl/mL resin
• Dynamic binding capacity: ~ 27 mg BSA/mL resin
• Recommended flow rate: < 600 cm/h
• Recommended column height: 100 mm
• pH working range: 3 to 13
• CIP stability: 2 to 14
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: 1. Wash with 3 column volumes (CV) of water or elution buffer.
  2. Wash with 4 CV 0.5 to 1.0 M NaOH at a low flow rate (≤ 0.78 mL/min (≤ 100 cm/h)).
  3. Wash with at least 3 CV water.
• Pack size: 1 × 4.7 mL
• Dimensions: 7.7 × 100 mm
• Column i.d.: 7.7 mm
• Column hardware pressure limit: 0.8 MPa (8 bar, 116 psi)