HiScreen Capto Q Column

• Cat#No#: Hi-214P
• Product Overview: HiScreen Capto Q is a column packed with a strong anion exchange modern resin, combining speed and capacity. Suitable for process development.
• Characteristic: Prepacked with Capto Q strong anion exchange chromatography resin.
  Convenient method optimization and parameter screening because of the 10cm bed height.
  These columns are easily connected in series to increase bed height to 20 cm.
  Fast results and minimal consumption of sample and buffer consumption through the small bed volume.
  BioProcess columns packed with the same chromatography resin and using the same linear fluid velocity can produce scalable, reproducible results.
• Maximum operating pressure: 3 bar [0.3 MPa] (44 psi)
• Sample preparation: 1. Adjust the sample to the composition of the start buffer, using one of these methods: Dilute the sample with start buffer. Exchange buffer using a HiPrep 26/10 Desalting, HiTrap Desalting or PD-10 Desalting column.
  2. Filter the sample through a 0.45 µm filter or centrifuge at 10 000 × g for 10 min immediately before loading it to the column. This prevents clogging and increases the life time of the column when loading large sample volumes.
• Metal ion capacity: 0.16 to 0.22 mmol Cl- /mL resin
• Matrix: Highly cross-linked agarose, spherical
• Ionic Exchanger Type: Strong anion, Q
• Average particle size: ~ 90µm
• Dynamic binding capacity: > 100 mg BSA/mL resin
• Recommended flow rate: < 700 cm/h
• Recommended column height: 100 mm
• Chemical stability: Stable to commonly used aqueous buffers, 1.0 M NaOH 8 M Urea, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol.
• pH working range: 2 to 12
• CIP stability: 2 to 14
• Temperature stability: 4°C to 30°C
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: 1. Wash with at least 2 column volumes (CV) of 2.0 M NaCl.
  2. Wash with at least 4 CV 1.0 M NaOH.
  3. Wash with at least 2 CV 2.0 M NaCl.
  4. Wash with at least 2 CV ultra pure water.
  5. Wash with Capto DEAE At least 10 CV start buffer or until eluent pH and conductivity have reached the required values.
• Pack size: 1 × 4.7 mL
• Maximum flow velocity: 700 cm/h
• Dimensions: 7.7 × 100 mm
• Column volume: 4.7 mL
• Column i.d.: 7.7 mm
• Column hardware pressure limit: 0.8 MPa (8 bar, 116 psi)
• Functional group: -N+ (CH3 )3