HiScreen Capto S ImpAct

• Cat#No#: Hi-217P
• Product Overview: HiScreen Capto S ImpAct columns are prepacked with Capto S ImpAct cation exchange chromatography medium, and are part of our process development platform. The columns are an excellent choice for method optimization and parameter screening.
• Description: HiScreen Capto S ImpAct (4.7 mL) are prepacked columns for small scale purifications, as well as optimization of methods and parameters, such as sample load and binding conditions. HiScreen Capto S ImpAct columns provide fast, reproducible and easy separations in a convenient format. The columns are best utilized when they are connected to liquid chromatography systems.
• Characteristic: 10 cm bed height of HiScreen columns is designed to allow method optimization and parameter screening.
  Small bed volume gives fast results and minimal sample/buffer consumption.
  Easily connected in series to achieve 20 cm bed height.
  Reproducible results, scalable to BioProcess columns packed with the same chromatography resins using the same linear fluid velocity. Prepacked with Capto S ImpAct, a strong cation exchange BioProcess resin, designed for immediate purification and polishing of a wide range of biomolecules, especially monoclonal antibodies.
• Sample preparation: 1. Adjust the sample to the composition of the start buffer, using one of these methods: Dilute the sample with start buffer. Exchange buffer using a HiPrep 26/10 Desalting, HiTrap Desalting or PD-10 Desalting column.
  2. Filter the sample through a 0.45 µm filter or centrifuge at 10 000 × g for 10 min immediately before loading it to the column. This prevents clogging and increases the life time of the column when loading large sample volumes.
• Metal ion capacity: 37 to 63 µmol (H+ ) /ml medium
• Matrix: High flow agarose
• Average particle size: 50 µm
• Dynamic binding capacity: > 90 mg lysozyme; > 85 mg BSA; > 100 mg IgG
• Recommended flow rate: 100 cm/h - 300 cm/h 1
• Recommended column height: 100 mm
• Chemical stability: All commonly used aqueous buffers, 1 M sodium hydroxide, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol and 70% ethanol.
• pH working range: 4 to 12
• CIP stability: 3 to 14
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: 1. Wash with at least 2 column volumes (CV) of 2.0 M NaCl.
  2. Wash with at least 4 CV 1.0 M NaOH.
  3. Wash with at least 2 CV 2.0 M NaCl.
  4. Wash with at least 2 CV ultra pure water.
  5. Wash with Capto DEAE At least 10 CV start buffer or until eluent pH and conductivity have reached the required values.
• Pack size: 1 × 4.7 mL
• Maximum flow velocity: At least 220 cm/h in a 1 m diameter column with bed height 20 cm at 20°C; measured using process buffers with the same viscosity as water at < 3 bar (0.3 MPa).
• Dimensions: 7.7 × 100 mm
• Column volume: 4.7 mL
• Column i.d.: 7.7 mm
• Column hardware pressure limit: 8 bar (0.8 MPa)
• Functional group: SO3 -