HiScreen Capto S

• Cat#No#: Hi-216P
• Product Overview: HiScreen Capto S columns are prepacked with Capto S ion exchange chromatography medium, and are part of the process development platform available. The columns are an excellent choice for method optimization and parameter screening.
• Description: The small column volume of 4.7 mL and bed height of 10 cm make HiScreen columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up.
• Characteristic: Capto S is a strong cation exchange BioProcess resin, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification.
  Prepacked, ready-to-use columns for convenient process development optimization.
  10 cm bed height of HiScreen columns is designed to allow method optimization and parameter screening.
  Easily connected in series to achieve 20 cm bed height.
  Small bed volume gives fast results and minimal sample/buffer consumption.
  Reproducible results, scalable to BioProcess columns packed with the same resin using the same linear fluid velocity.
• Maximum operating pressure: 3 bar [0.3 MPa] (44 psi)
• Sample preparation: 1. Adjust the sample to the composition of the start buffer, using one of these methods: Dilute the sample with start buffer. Exchange buffer using a HiPrep 26/10 Desalting, HiTrap Desalting or PD-10 Desalting column.
  2. Filter the sample through a 0.45 µm filter or centrifuge at 10 000 × g for 10 min immediately before loading it to the column. This prevents clogging and increases the life time of the column when loading large sample volumes.
• Metal ion capacity: 0.11 to 0.14 mmol Na+ /mL resin
• Matrix: Highly cross-linked agarose, spherical
• Ionic Exchanger Type: Strong cation, S
• Average particle size: ~ 90µm
• Dynamic binding capacity: > 120 mg lysozyme/ml medium
• Recommended flow rate: < 700 cm/h
• Recommended column height: 100 mm
• Chemical stability: Stable to commonly used aqueous buffers, 1.0 M NaOH 8 M Urea, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol.
• pH working range: 4 to 12
• CIP stability: 3 to 14
• Storage: 4 to 30°C, 0.2 M Sodium Acetate in 20% Ethanol.
• Cleaning-in-place: 1. Wash with at least 2 column volumes (CV) of 2.0 M NaCl.
  2. Wash with at least 4 CV 1.0 M NaOH.
  3. Wash with at least 2 CV 2.0 M NaCl.
  4. Wash with at least 2 CV ultra pure water.
  5. Wash with Capto DEAE At least 10 CV start buffer or until eluent pH and conductivity have reached the required values.
• Pack size: 1 × 4.7 mL
• Maximum flow velocity: 700 cm/h
• Dimensions: 7.7 × 100 mm
• Column volume: 4.7 mL
• Column i.d.: 7.7 mm
• Column hardware pressure limit: 0.8 MPa (8 bar, 116 psi)
• Functional group: -SO3 -