HiScreen Capto SP Impres Column

• Cat#No#: Hi-218P
• Product Overview: HiScreen Capto SP ImpRes column is packed with strong cation exchange modern resin for fast high resolution protein purification in process development.
• Characteristic: Prepacked with Capto SP strong cation exchange chromatography resin, suitable for intermediate protein purification and large-scale polishing.
  Convenient method optimization and parameter screening because of the 10 cm bed height.
  These columns are easily connected in series to increase bed height to 20 cm.
  Fast results and minimal consumption of sample and buffer because of the small bed volume.
  BioProcess columns packed with the same chromatography resin and using the same linear fluid velocity can produce scalable, reproducible results.
• Maximum operating pressure: 4 bar [0.4 MPa] (59 psi)
• Sample preparation: 1. Adjust the sample to the composition of the start buffer, using one of these methods: Dilute the sample with start buffer. Exchange buffer using a HiPrep 26/10 Desalting, HiTrap Desalting or PD-10 Desalting column.
  2. Filter the sample through a 0.45 µm filter or centrifuge at 10 000 × g for 10 min immediately before loading it to the column. This prevents clogging and increases the life time of the column when loading large sample volumes.
• Metal ion capacity: 0.13 to 0.16mmol H+ /ml medium
• Matrix: High flow agarose
• Particle Size: 36 to 44 µm
• Dynamic binding capacity: >70 mg lysozyme/ml medium; >95 mg BSA/ml medium.
• Recommended flow rate: 100 cm/h - 300 cm/h
• Recommended column height: 100 mm
• Chemical stability: All commonly used aqueous buffers, 1 M NaOH5 , 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol and 70% ethanol.
• pH working range: 4 to 12
• CIP stability: 3 to 14
• Storage: 4 to 30°C, 0.2 M Sodium Acetate in 20% Ethanol.
• Cleaning-in-place: 1. Wash with at least 2 column volumes (CV) of 2.0 M NaCl.
  2. Wash with at least 4 CV 1.0 M NaOH.
  3. Wash with at least 2 CV 2.0 M NaCl.
  4. Wash with at least 2 CV ultra pure water.
  5. Wash with Capto DEAE At least 10 CV start buffer or until eluent pH and conductivity have reached the required values.
• Pack size: 1 × 4.7 mL
• Maximum flow velocity: 400 cm/h
• Dimensions: 7.7 × 100 mm
• Column volume: 4.7 mL
• Column i.d.: 7.7 mm
• Column hardware pressure limit: 8 bar (0.8 MPa)
• Functional group: -CH2CH2CH2SO3 -