HiScreen IMAC FF

• Cat#No#: Hi-066P
• Product Overview: HiScreen IMAC FF are prepacked with IMAC Sepharose 6 Fast Flow, a resin which is charged with the metal of choice for purification of histidine-tagged proteins. The HiScreen format is optimized for method optimization and parameter screening.
• Description: IMAC Sepharose 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose to which a chelating group has been covalently coupled. This chelating group can be charged with suitable metal ions, specific for your target protein of interest.
• Characteristic: For optimizing purification of histidine-tagged proteins when Ni2+ is not the best choice of metal ion.
  Conveniently charge with your metal ion of choice.
  Optimal bead size for scale-up.
  High binding capacity.
  Prepacked HiScreen columns for method optimization and process development.
  Reproducible results, scalable to BioProcess columns packed with the same resin using the same linear fluid velocity.
• Maximum operating pressure: 1.5 bar [0.15 MPa] (22 psi)
• Metal ion capacity: Approx. 15 µmol Ni2+ /ml medium
• Matrix: Highly cross-linked 6% agarose
• Average particle size: 90 µm
• Dynamic binding capacity: Approx. 40 mg (histidine)6-tagged protein/ml medium (Ni2+-charged). Untagged protein: Approx. 25 mg/ml medium (Cu2+ charged), or approx. 15 mg/ml medium (Zn2+ or Ni2+ charged).
• Recommended flow rate: 30 to 300 cm/h
• Recommended column height: 100 mm
• Chemical stability: 1 M NaOH, 70% acetic acid. Tested for 12 h. 2% SDS. Tested for 1 h. 30% 2-propanol. Tested for 30 min.
• pH working range: 3 to 12
• CIP stability: 2 to 14
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: Ionically bound proteins: 1. Wash with several column volumes (CV) of 1.5 M NaCl. 2. Wash with at least 3 CV distilled water.
  Precipitated proteins, hydrophobically bound proteins, and lipoproteins: 1. Wash the column with 1 M NaOH, contact time usually 1 to 2 h (longer time may be required to inactivate endotoxins). 2. Wash with approximately 3 to 10 CV start buffer. 3. Wash with 5 to 10 CV distilled water.
  Hydrophobically bound proteins, lipoproteins, and lipids: 1. Wash with 5 to 10 CV 30% isopropanol for at least 15 to 20 min. 2. Wash with approximately 10 CV distilled water. or 1. Wash with 2 CV detergent in a basic or acidic solution. Use, for example, 0.1 to 0.5% nonionic detergent in 0.1 M acetic acid, contact time 1 to 2 h. 2. Remove residual detergent by washing with at least 5 CV 70% ethanol. 3. Wash with 3 to 10 CV start buffer.
• Pack size: 4.7 mL
• Maximum flow velocity: 600 cm/h
• Dimensions: 7.7 × 100 mm
• Column volume: 4.7 ml
• Column i.d.: 7.7 mm
• Column hardware pressure limit: 0.8 MPa (8 bar, 116 psi)