HiScreen IXSelect

• Cat#No#: Hi-067P
• Product Overview: HiScreen IXSelect columns are prepacked with IXSelect affinity chromatography medium, and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.
• Description: IXSelect is an affinity chromatography resin designed for selective binding of coagulation Factor IX (FIX). The resin can be used, under mild elution conditions, for purifying FIX from plasma as well as from recombinant sources.
• Characteristic: IXSelect is a high-throughput resin specifically designed for the purification of factor IX targets from recombinant feed streams.
  10 cm bed height of HiScreen columns is designed to allow method optimization and parameter screening.
  Easily connected in series to achieve 20 cm bed height.
  Small bed volume gives fast results and minimal sample/buffer consumption.
  Reproducible results, scalable to BioProcess columns packed with the same chromatography resin using the same linear fluid velocity.
• Matrix: Highly cross-linked agarose, spherical
• Average particle size: 75 μm
• Ligand: Recombinant protein (Mr 13 151) produced in Saccharomyces cerevisiae
• Ligand density: Approx. 8 mg/mL resin
• Dynamic binding capacity: Approx. 6 mg FIX/mL resin
• Recommended flow rate: Minimum 600 cm/h in a 1 m diameter column, with 20 cm bed height at 20˚C using buffers with the same viscosity as water at < 0.3 MPa (3 bar)
• Recommended column height: 20 cm
• pH working range: 3 to 10
• CIP stability: 2 to 12
• Storage: 4˚C to 30˚C
• Pack size: 1 × 4.7 mL
• Wash buffer: 20 mM Tris-HCl, 500 mM NaCl, 0.01% Tween 80, pH 7.4