HiScreen MabSelect SuRe LX protein A column

• Cat#No#: Hi-009P
• Product Overview: The HiScreen MabSelect SuRe LX column uses protein A chromatography for antibody purification. This protein A column is prepacked with MabSelect SuRe LX affinity resin and is part of our process development platform. These packed columns are an excellent choice for method optimization and parameter screening for capture of monoclonal antibodies when a high dynamic binding capacity is needed.
• Description: MabSelect SuRe LX is an alkali-stabilized, protein A-derived affinity medium with a binding capacity for monoclonal antibodies (MAbs) exceeding that of MabSelect SuRe at longer residence times. This special combination of high binding capacity plus alkaline stability gives manufacturers of MAbs many opportunities to improve process economics and product quality.
  High dynamic binding capacity (DBC) helps users process feed from high-expression cell cultures with increased antibody titers in shorter time or with smaller chromatography unit operations. Enhanced alkaline stability means that regular cleaning-in-place (CIP) can be performed with cost-effective agents such as sodium hydroxide (NaOH) to prolong the medium's working lifetime.
• Characteristic: The alkali-tolerant recombinant protein A ligand of MabSelect Sure LX allows clean-in-place (CIP) and the resin gives excellent dynamic binding capacity.
  Prepacked, ready-to-use columns for convenient chromatography process development.
  10 cm bed height of HiScreen columns is designed to allow method optimization and parameter screening.
  Easily connected in series to achieve 20 cm bed height.
  Small bed volume gives fast results and minimal sample/buffer consumption.
  Reproducible results, scalable to BioProcess columns packed with the same chromatography resin using the same linear fluid velocity.
• Maximum operating pressure: 3 bar [0.3 MPa] (44 psi)
• Ligand Coupling Method: Single-point attachment
• Matrix: Rigid, highly cross-linked agarose
• Average particle size: 85 µm
• Ligand: Alkali-stabilized, protein A-derived (E. coli)
• Coupling chemistry: Epoxy
• Dynamic binding capacity: Approx 60 mg human IgG/ml medium at 6 min residence time.
• Recommended flow rate: < 500 cm/h
• Recommended column height: 100 mm
• Chemical stability: Stable in all aqueous buffers commonly used in protein A chromatography.
• pH working range: 3–12
• CIP stability: 3 to 13.7
• Temperature stability: 2°C to 40°C
• Storage: 2 to 8°C, 20% Ethanol
• Shipping: 20% ethanol
• Cleaning-in-place: 1. Wash the column with 3 column volumes (CV) of start buffer.
  2. Wash with at least 2 CV 0.1 to 0.5 M NaOH with a contact time of 10 to 15 minutes.
  3. Wash immediately with at least 5 CV start buffer at pH 7 to 8.
• Pack size: 1 × 4.7 mL
• Maximum flow velocity: 500 cm/h
• Dimensions: 7.7 × 100 mm
• Column volume: 4.7 ml
• Column i.d.: 7.7 mm
• Column hardware pressure limit: 0.8 MPa (8 bar)