HiScreen MabSelect Xtra

• Cat#No#: Hi-011P
• Product Overview: HiScreen MabSelect Xtra is an excellent choice for method optimization and parameter screening for capture of monoclonal antibodies (MAbs).
• Description: MabSelect Xtra is a recombinant protein A-based affinity medium engineered to give an exceptionally high dynamic binding capacity for monoclonal antibodies. In addition, MabSelect Xtra is optimized for Fc-fusion proteins. Higher capacity translates directly to lower cost of production.
• Characteristic: High dynamic binding capacity and suitable for highexpression feedstocks.
  Improved process economics through reduced raw materials costs and/or reduced number of cycles.
  Very low, unspecific binding due to high ligand selectivity and matrix hydrophilicity.
  High capacity for many Fc-fusion proteins.
• Maximum operating pressure: 3 bar [0.3 MPa] (44 psi)
• Average particle size: 75 µm
• Ligand: Recombinant protein A (E. coli).
• Coupling chemistry: Epoxy
• Dynamic binding capacity: ~ 40 mg human IgG/ml medium
• Recommended flow rate: 100–300 cm/h
• Recommended column height: 100 mm
• Chemical stability: Stable in all aqueous buffers commonly used in protein A chromatography: 0.1 M 20% sodium citrate/HCl (pH 3), 6 M Gua-HCl, 6 M urea, 20% ethanol, 2% benzyl alcohol
• pH working range: 3–10
• CIP stability: 2–12
• Temperature stability: 2°C–40°C
• Storage: 2°C to 8°C in 20% ethanol.
• Shipping: 20% ethanol
• Cleaning-in-place: Precipitated or denatured substances: 1. Wash with 2 column volumes (CV) of 6 M guanidine hydrochloride, contact time at least 10 min. 2. Wash immediately with at least 5 CV filtered start buffer. or 1. Wash with 2 CV 50 mM NaOH of 1.0 M NaCl or 50 mM NaOH in 0.5 M Na2SO4 , contact time ~ 10 min. 2. Wash immediately with at least 5 CV filtered start buffer.
  Hydrophobically bound substances: 1. Wash with 2 CV 50 mM NaOH 1.0 M NaCl or 50 mM NaOH in 0.5 M Na2SO4 , contact time ~ 10 min. Wash with 2 CV nonionic detergent (e.g., conc. 0.1%), contact time ~ 10 min. 2. Wash immediately with at least 5 CV start buffer. or 1. Wash with 3 to 4 CV 70% ethanol or 30% isopropanol, contact time ~ 10 min. Increasing gradients may be applied to avoid air bubble formation when using high concentrations of organic solvents. 2. Wash immediately with at least 5 CV start buffer.
• Sanitization: 1. Wash the column with 0.1 M acetic acid in 20% ethanol. 2. Leave the column in contact with the solution for 1 hour. 3. Wash with at least 5 CV sterile start buffer at pH 7 to 8. or 1. Wash the column with 70% ethanol. 2. Leave the column in contact with the solution for 12 hours. 3. Wash with at least 5 CV sterile start buffer at pH 7 to 8.
• Pack size: 1 × 4.7 mL
• Maximum flow velocity: 300 cm/h
• Dimensions: 7.7 × 100 mm
• Column volume: 4.7 ml
• Column i.d.: 7.7 mm
• Column hardware pressure limit: 0.8 MPa (8 bar)