HiScreen Ni FF

• Cat#No#: Hi-100P
• Product Overview: HiScreen columns prepacked with Ni Sepharose 6 Fast Flow for method optimization and parameter screening for purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC).
• Description: The columns are prepacked with a range of BioProcess chromatography resins (resin) and designed for method optimization and parameter screening. HiScreen columns have small bed volumes (4.7 mL), reducing the cost of sample and buffer consumption. The chromatography resins used in HiScreen columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.
• Characteristic: High binding capacity, approx. 40 mg/mL resin and negligible leakage of Ni2+.
  Prepacked, ready-to-use columns for convenient process development.
  Excellent choice for method optimization and parameter screening due to the 10 cm bed height.
  Easy connection in series to achieve 20 cm bed height.
  Small bed volume gives fast results and minimal sample/buffer consumption.
  Reproducible results, scalable to BioProcess columns packed with the same resin using the same linear fluid velocity.
• Maximum operating pressure: 1.5 bar [0.15 MPa] (22 psi)
• Metal ion capacity: Approx. 15 µmol Ni2+ /ml medium
• Matrix: Highly cross-linked 6% agarose
• Average particle size: 90 µm
• Dynamic binding capacity: Approx. 40 mg (histidine)6-tagged protein/ml medium (Ni2+-charged).
• Recommended flow rate: < 450 cm/h
• Recommended column height: 100 mm
• Chemical stability: 1 M NaOH, 70% acetic acid; tested for 12 h. 2% SDS; tested for 1 h. 30% 2-propanol.; tested for 30 min.
• pH working range: 3 to 12
• CIP stability: 2 to 14
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: Ionically bound proteins: 1. Wash with several column volumes (CV) of 1.5 M NaCl. 2. Wash with at least 3 CV distilled water. Precipitated proteins, hydrophobically bound proteins, and lipoproteins: 1. Wash the column with 1 M NaOH, contact time usually 1 to 2 h (longer time may be required to inactivate endotoxins). 2. Wash with approximately 3 to 10 CV binding buffer. 3. Wash with 5 to 10 CV distilled water.
  Hydrophobically bound proteins, lipoproteins, and lipids: 1. Wash with 5 to 10 CV 30% isopropanol for at least 15 to 20 min. 2. Wash with approximately 10 CV distilled water. or 1. Wash with 2 CV detergent in a basic or acidic solution. Use, for example, 0.1% to 0.5% nonionic detergent in 0.1 M acetic acid, contact time 1 to 2 h. 2. Remove residual detergent by washing with at least 5 CV 70% ethanol1 . 3. Wash with 3 to 10 CV binding buffer.
• Pack size: 4.7 mL
• Maximum flow velocity: 450 cm/h
• Dimensions: 7.7 × 100 mm
• Column volume: 4.7 ml
• Column i.d.: 7.7 mm
• Column hardware pressure limit: 8 bar (0.8 MPa)