HisPrep FF

• Cat#No#: His-109P
• Product Overview: HisPrep FF 16/10 is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow. The column is an excellent choice for preparative, scale-up purification of histidine-tagged recombinant proteins.
• Characteristic: Optimized for convenient scale-up purification of histidine-tagged proteins.
  Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
  Negligible leakage of Ni2+.
  High binding capacity, approx. 40 mg/mL resin.
  Convenient and time-saving 20 mL prepacked HiPrep format.
• Metal ion capacity: Approx. 15 μmol Ni2+ /ml medium
• Matrix: Highly cross-linked spherical agarose, 6%
• Average particle size: 90 μm
• Dynamic binding capacity: Approx. 40 mg (histidine)6 -tagged protein/ml medium.
• Recommended flow rate: 1 to 10 ml/min (30 to 300 cm/h)
• Chemical stability: 1 M NaOH, 70% acetic acid; tested for 12 hours. 2% SDS; tested for 1 hour. 30% 2- propanol; tested for 30 minutes.
• Chemical compatibility: Stable in all commonly used buffers, reducing agents, denaturants, and detergents.
• pH working range: 3 to 12
• CIP stability: 2 to 14
• Storage: 4°C to 30°C in 20% ethanol
• Shipping: 20% ethanol
• Cleaning-in-place: Ionically bound proteins: 1. Wash with several column volumes (CV) of 1.5 M NaCl. 2. Wash with at least 3 CV distilled water. Precipitated proteins, hydrophobically bound proteins, and lipoproteins: 1. Wash the column with 1 M NaOH, contact time usually 1 to 2 h (longer time may be required to inactivate endotoxins). 2. Wash with approximately 3 to 10 CV binding buffer. 3. Wash with 5 to 10 CV distilled water.
  Hydrophobically bound proteins, lipoproteins, and lipids: 1. Wash with 5 to 10 CV 30% isopropanol for at least 15 to 20 min. 2. Wash with approximately 10 CV distilled water. or 1. Wash with 2 CV detergent in a basic or acidic solution. Use, for example, 0.1 to 0.5% nonionic detergent in 0.1 M acetic acid, contact time 1 to 2 h. 2. Remove residual detergent by washing with at least 5 CV 70% ethanol1 . 3. Wash with 3 to 10 CV binding buffer.
• Pack size: 20 mL
• Maximum flow velocity: 10 ml/min (300 cm/h)
• Column hardware pressure limit: 5 bar (0.5 MPa)