HisTrap FF Crude columns

• Cat#No#: His-111P
• Product Overview: HisTrap FF Crude columns are high binding capacity IMAC columns for his-tagged protein purification when scalability is needed.
• Characteristic: High binding capacity – binds up to 40 mg his-tag protein/mL resin.
  Excellent flow properties – great performance even at high flow rates.
  Optimized for unclarified samples – short purification time can minimize potential negative effects, such as degradation and oxidation of proteins.
  HiTrap column format – compatible with syringe, pump, or chromatography system such as ÄKTA systems or other FPLC systems.
• Applications: Intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Metal ion capacity: ~ 15 μmol Ni2+ /ml medium
• Matrix: Highly cross-linked spherical agarose, 6%
• Average particle size: 90 μm
• Dynamic binding capacity: Approx. 40 mg histidine-tagged protein/ml medium.
• Recommended flow rate: 1 ml/min and 5 ml/min for 1 ml and 5 ml column, respectively.
• Recommended column height: 25 mm
• Chemical stability: 0.01 M HCl, 0.1 M NaOH; Tested for one week at 40°C. 1 M NaOH, 70% acetic acid; Tested for 12 h. 2% SDS; Tested for 1 h. 30% 2-propanol; Tested for 30 min.
• Chemical compatibility: Stable in all commonly used buffers, reducing agents, denaturants, and detergents.
• pH working range: 2 to 14
• CIP stability: 3 to 12
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: Ionically bound proteins: Wash with several column volumes of 1.5 M NaCl, then wash with approx. 10 column volumes of distilled water.
  Precipitated proteins, hydrophobically bound proteins, and lipoproteins: Wash the column with 1 M NaOH, contact time usually 1 to 2 h (12 h or more for endotoxin removal). Then wash with approx. 10 column volumes of binding buffer, followed by 5 to 10 column volumes of distilled water.
  Hydrophobically bound proteins, lipoproteins, and lipids: Wash with 5 to 10 column volumes of 30% isopropanol for about 15 to 20 min. Then wash with approx. 10 column volumes of distilled water. Alternatively, wash with 2 column volumes of detergent in a basic or acidic solution. Use, for example, 0.1 to 0.5% nonionic detergent in 0.1 M acetic acid, contact time 1 to 2 h. After treatment, always remove residual detergent by washing with at least 5 column volumes of 70% ethanol1 . Then wash with approx. 10 column volumes of distilled water.
• Pack size: 5 × 1 mL
• Maximum flow velocity: 4 ml/min and 20 ml/min for 1 ml and 5 ml column, respectively.
• Dimensions: 7 × 25 mm
• Column volume: 1 ml
• Column i.d.: 7 mm
• Column hardware pressure limit: 5 bar (0.5 MPa)