HisTrap HP columns

• Cat#No#: His-112P
• Product Overview: HisTrap HP prepacked columns provide a fast, convenient and reproducible format for preparative purification of histidine-tagged (His-tag) recombinant proteins.
• Characteristic: High resolution: due to small bead size (average bead size is 34 µm).
  High binding capacity: at least 40 mg His tag protein per mL resin, for high yields.
  Negligible nickel leakage: helps retain activity and minimize protein precipitation.
  Broad reagent compatibility: suitable for use with a wide range of reducing agents, detergents, denaturants, and other additives. Convenient HiTrap format: 1 mL and 5 mL columns compatible with syringe, pump, or chromatography systems.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Metal ion capacity: ~ 15 μmol Ni2+ /ml medium
• Matrix: Highly cross-linked spherical agarose, 6%
• Average particle size: 34 μm
• Dynamic binding capacity: At least 40 mg (histidine)6 -tagged protein/ml medium.
• Recommended flow rate: 1 ml/min and 5 ml/min for 1 ml and 5 ml column, respectively.
• Recommended column height: 25 mm
• Chemical stability: 0.01 M HCl, 0.1 M NaOH; tested for one week at 40°C. 1 M NaOH, 70% acetic acid; tested for 12 h. 2% SDS; tested for 1 h. 30% 2-propanol; tested for 30 min.
• Chemical compatibility: Stable in all commonly used buffers, reducing agents, denaturants, and detergents.
• pH working range: 3 to 12
• CIP stability: 2 to 14
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: Ionically bound proteins: Wash with several column volumes of 1.5 M NaCl; then wash with approx. 10 column volumes of distilled water.
  Precipitated proteins, hydrophobically bound proteins, and lipoproteins: Wash the column with 1 M NaOH, contact time usually 1 to 2 hours (12 hours or more for endotoxin removal). Then wash with approx. 10 column volumes of binding buffer, followed by 5 to 10 column volumes of distilled water.
  Hydrophobically bound proteins, lipoproteins, and lipids: Wash with 5 to 10 column volumes of 30% iso-propanol for about 15 to 20 minutes. Then wash with approx. 10 column volumes of distilled water. Alternatively, wash with 2 column volumes of detergent in a basic or acidic solution. Use, for example, 0.1 to 0.5% nonionic detergent in 0.1 M acetic acid, contact time 1 to 2 hours. After treatment, always remove residual detergent by washing with at least 5 column volumes of 70% ethanol1 . Then wash with approx. 10 column volumes of distilled water.
• Pack size: 5 × 1 mL
• Maximum flow velocity: 4 ml/min and 20 ml/min for 1 ml and 5 ml column, respectively.
• Dimensions: 7 × 25 mm
• Column volume: 1 ml
• Column i.d.: 7 mm
• Column hardware pressure limit: 5 bar (0.5 MPa)