HiTrap Benzamidine FF (High Sub)

• Cat#No#: Hi-069P
• Product Overview: HiTrap Benzamidine FF (high sub) are prepacked with Benzamidine Sepharose 4 Fast Flow (high sub) and designed specifically for the removal and/or purification of serine proteases such as thrombin.
• Description: HiTrap Benzamidine FF (high sub) are prepacked 1 mL and 5 mL columns for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein. Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.
• Characteristic: Effective removal of thrombin and factor Xa after tag cleavage of recombinant proteins.
  Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.
  HiTrap Benzamidine FF for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.
  High binding capacity.
  The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTA design or FPLC System.
• Applications: For the removal and/or purification of serine proteases e.g. thrombin.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Matrix: Highly cross-linked agarose, 4%
• Average particle size: 90 μm
• Ligand: p-aminobenzamidine (pABA)
• Ligand density: 12 μmol p-aminobenzamidine/ml medium
• Dynamic binding capacity: 35 mg trypsin/ml medium
• Recommended flow rate: < 4 ml/min
• Recommended column height: 25 mm
• Chemical stability: All commonly used aqueous buffers
• pH working range: 1 to 9
• CIP stability: 2 to 8
• Storage: 2 to 8°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol.
• Pack size: 5 × 1 mL
• Maximum flow velocity: 4 ml/min and 20 ml/min for 1 and 5 ml columns respectively.
• Dimensions: 7 × 25 mm
• Column volume: 1 mL
• Column i.d.: 7 mm
• Column hardware pressure limit: 5 bar (0.5 MPa)