| Cat# | Product Name | Size | Price | Qty | Inquiry |
|---|---|---|---|---|---|
| Hi-070P | HiTrap Blue HP | Inquiry |
| Product Information | |
| Cat#No# | Hi-070P |
| Product Overview | HiTrap Blue HP is prepacked with Blue Sepharose High Performance for capture of a wide range of biomolecules, including: albumin, interferon, a broad range of nucleotide-dependent enzymes, α2-macroglobulin, coagulation factors, and related proteins. |
| Description | HiTrap Blue HP is one of a range of prepacked columns for affinity chromatography. Fast, simple and easy separations are provided by the combination of a specially designed column and a high performance affinity medium. HiTrap Blue HP is particularly suitable for the isolation and purification of albumin, interferon, a broad range of nucleotide requiring enzymes, a2 -macroglobulin, coagulation factors, and nucleic acid binding proteins. The removal of albumin is a particularly important application since excess amounts of albumin can disturb the results of several tests. |
| Characteristic | Fast and convenient use. Prepacked with Blue Sepharose High Performance. Simple operation with a syringe, a pump, an ÄKTA system, or other chromatography systems. |
| Maximum operating pressure | 5 bar [0.5 MPa] (70 psi) |
| Sample preparation | The sample should be adjusted to the composition of the binding buffer. This can be done by either diluting the sample with binding buffer or by buffer exchange using HiTrap Desalting, PD-10 column or a HiPrep 26/10 Desalting column depending on the sample volume. The sample should be filtered through a 0.45 μm filter or centrifuged immediately before it is applied to the column. |
| Matrix | Highly cross-linked, spherical agarose |
| Average particle size | 34 µm |
| Ligand | Cibacron Blue F3G-A |
| Ligand density | 4 mg/ml medium |
| Dynamic binding capacity | 20 mg human albumin/ml medium |
| Recommended flow rate | < 4 ml/min |
| Recommended column height | 25 mm |
| Chemical stability | All commonly used buffers, 70% ethanol, 8 M urea and 6 M guanidine hydrochloride. |
| pH working range | 4 to 12 |
| CIP stability | 3 to 13 |
| Temperature stability | 4°C to room temperature |
| Storage | 4 to 30°C, 20% Ethanol |
| Pack size | 5 × 1 mL |
| Maximum flow velocity | 4 ml/min and 20 ml/min for 1 ml and 5 ml column respectively. |
| Dimensions | 7 × 25 mm |
| Column volume | 1 mL |
| Column i.d. | 7 mm |
| Column hardware pressure limit | 5 bar (70 psi, 0.5 MPa) |
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For Research or Industrial Raw Materials, Not For Personal Medical Use!Online Inquiry
