HiTrap Butyl HP

• Cat#No#: Hi-148P
• Product Overview: HiTrap Butyl HP are prepacked Butyl Sepharose High Performance columns for high-resolution hydrophobic interaction chromatography (HIC). The columns are used for small-scale protein purification as well as for screening of binding and elution conditions.
• Characteristic: Provide convenient and fast small-scale protein purifications.
  Butyl Sepharose High Performance is an aliphatic HIC resin, designed for intermediate and polishing step where high resolution has priority.
  Flexible design for use with syringes, single laboratory pump, or chromatography system.
  High flow rates and high capacities.
  Excellent and easy scale-up achieved by connecting several HiTrap columns in series or using HiPrep columns.
• Applications: For screening of selectivity, binding and elution conditions, as well as small scale purifications.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Matrix: cross-linked agarose, spherical
• Average particle size: ~34 µm
• Ligand density: ~ 50 µmol Butyl/mL resin
• Dynamic binding capacity: ~ 38 mg β-lactoglobulin/mL resin
• Recommended flow rate: ≤ 100 cm/h
• Recommended column height: 25 mm
• Chemical stability: Stable to commonly used aqueous buffers, 0.01 M NaOH, 1.0 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% acetonitrile, 70% ethanol, 1 mM HCl, 30% isopropanol, 3 M (NH4 )SO4 5 , and 2 % SDS.
• pH working range: 3 to 12
• CIP stability: 2 to 14
• Storage: 4°C to 30°C, 20% Ethanol
• Pack size: 5 × 1 mL
• Maximum flow velocity: 4.0 mL/min (1 mL), 20 mL/min (5 mL)
• Dimensions: 7 × 25 mm
• Column i.d.: 7 mm
• Column hardware pressure limit: 5 bar (0.5 MPa, 73 psi)
• Functional group: Butyl —O—(CH2 ) 3 —CH3