HiTrap Capto adhere ImpRes

• Cat#No#: Hi-292P
• Product Overview: HiTrap Capto adhere ImpRes is a ready-to-use column pre-packed with BioProcess Capto adhere ImpRes strong anion exchange multimodal resin for screening and small-scale protein purifications using ion exchange chromatography (IEX).
• Description: The multimodal functionality of Capto adhere ImpRes gives a different selectivity compared with traditional ion exchange columns and it binds proteins at high or low ionic strength.
• Characteristic: High yields achieved through the high-resolution beads and selectivity of the ligand.
  Efficient removal of aggregates, viruses, and main contaminants in MAb processes.
  Columns for screening of selectivity, binding, and elution conditions, as well as for small scale purifications.
• Maximum operating pressure: 5 bar (0.5 Mpa, 70 psi)
• Sample preparation: 1. Adjust the sample to the composition of the start buffer, using one of these methods: Dilute the sample with start buffer. Exchange buffer using a HiPrep 26/10 Desalting, HiTrap Desalting or PD-10 Desalting column.
  2. Filter the sample through a 0.45 µm filter or centrifuge at 10 000 × g for 10 min immediately before loading it to the column. This prevents clogging and increases the life time of the column when loading large sample volumes.
• Metal ion capacity: 0.08 to 0.11 mmol Cl- /mL medium
• Matrix: highly cross-linked agarose
• Ionic Exchanger Type: multimodal strong anion exchanger
• Average particle size: 36 to 44 µm
• Recommended flow rate: 25 to 50 cm/h
• Recommended column height: 25 mm
• Chemical stability: All commonly used aqueous buffers, 1 M acetic acid, 1 M sodium hydroxide.
• pH working range: 3 to 12
• CIP stability: 2 to 14
• Storage: 4°C to 30°C in 20% ethanol
• Cleaning-in-place: 1. Wash with at least 2 column volumes (CV) of 2 M NaCl.
  2. Wash with at least 3 CV 1 M NaOH with at least 15 min contact time.
  3. Wash with at least 2 CV 2 M NaCl.
  4. Wash with at least 2 CV distilled water.
  5. Wash with 5 CV start buffer or until eluent pH and conductivity have reached the required values.
• Pack size: 5 × 1 mL
• Maximum flow velocity: 300 cm/h
• Dimensions: 7 × 25 mm
• Column volume: 1 mL
• Column i.d.: 7 mm
• Column hardware pressure limit: 5 bar (0.5 MPa)