HiTrap Capto Butyl ImpRes

• Cat#No#: Hi-151P
• Product Overview: HiTrap Capto Butyl ImpRes is a ready-to-use column prepacked with BioProcess Capto Butyl ImpRes resins for screening and small-scale protein purification using hydrophobic interaction chromatography.
• Description: Capto Butyl ImpRes is hydrophobic interaction chromatography (HIC) media (resins) developed for the intermediate and polishing steps in a downstream protein purification process. Both chromatography media extend the well established Capto platform to include high-resolution media. By combining the high-flow characteristics of Capto media with a smaller particle size, Capto Butyl ImpRes delivers excellent pressure/flow properties and resolution. The ability to run at higher flow velocities and higher bed heights increases flexibility in process design and might enable increased productivity.
• Characteristic: Prepacked columns for screening of selectivity, binding and elution conditions, as well as small scale purifications.
  High-throughput chromatography resin for intermediate and polishing steps in a downstream protein purification process.
  Excellent chemical stability allows cleaning with all standard CIP solutions, including 1 M sodium hydroxide.
  High binding capacity, high flow rate, and low back pressure reduces process cycle times and improves productivity at process scale.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Matrix: High-flow agarose
• Average particle size: 40 μm
• Ligand: Butyl
• Dynamic binding capacity: 37 mg BSA/mL medium
• Recommended flow rate: Up to 220 cm/h in a 1 m diameter column with a bed height of 20 cm at 20°C; measured using process buffers with the same viscosity as water at 300 kPa.
• Recommended column height: 25 mm
• pH working range: 3 to 13
• CIP stability: 2 to 14
• Shelf life: Five years
• Storage: 20% ethanol at 4°C to 30°C
• Pack size: 5 × 1 mL
• Dimensions: 7 × 25 mm
• Column i.d.: 7 mm