Product Information | |
Cat#No# | Hi-287P |
Product Overview | Novel core bead technology and multimodal ligand give Capto Core 400 dual functionality, size exclusion and binding chromatography, in one chromatography resin. |
Description | Capto Core 400 is an excellent alternative to size‑exclusion chromatography (SEC) resins that are typically employed in the final stages of virus purification in vaccine manufacture. |
Characteristic | High capacity and productivity in flowthrough mode. Prepacked HiTrap columns for easy screening and convenient process development, as well as small scale purification. Wide operational window of pH and conductivity. |
Maximum operating pressure | 5 bar (0.5 Mpa, 70 psi) |
Sample preparation | 1. Adjust the sample to the composition of the start buffer, using one of these methods: Dilute the sample with start buffer. Exchange buffer using a HiPrep 26/10 Desalting, HiTrap Desalting or PD-10 Desalting column. 2. Filter the sample through a 0.45 µm filter or centrifuge at 10 000 × g for 10 min immediately before loading it to the column. This prevents clogging and increases the life time of the column when loading large sample volumes. |
Matrix | Highly cross-linked agarose |
Average particle size | 90 µm |
Ligand | Octylamine |
Dynamic binding capacity | ~22 mg ovalbumin/mL resin |
Chemical stability | Stable in commonly used aqueous buffers, 1 M NaOH, 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol. |
pH working range | 3 to 13 |
CIP stability | 3 to 14 |
Storage | 4°C to 30°C, 20% ethanol |
Cleaning-in-place | Wash with a solution of 1 M NaOH in 30% isopropanol or in 27% 1-propanol with reversed flow direction. |
Pack size | 5 × 1 mL |
Maximum flow velocity | 700 cm/h |
Column hardware pressure limit | 0.5 MPa (5 bar, 72 psi) |
Download Datasheet |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Online Inquiry