Cat# | Product Name | Size | Price | Qty | Inquiry |
---|---|---|---|---|---|
Hi-287P | HiTrap Capto Core 400 | Inquiry |
Product Information | |
Cat#No# | Hi-287P |
Product Overview | Novel core bead technology and multimodal ligand give Capto Core 400 dual functionality, size exclusion and binding chromatography, in one chromatography resin. |
Description | Capto Core 400 is an excellent alternative to size‑exclusion chromatography (SEC) resins that are typically employed in the final stages of virus purification in vaccine manufacture. |
Characteristic | High capacity and productivity in flowthrough mode. Prepacked HiTrap columns for easy screening and convenient process development, as well as small scale purification. Wide operational window of pH and conductivity. |
Maximum operating pressure | 5 bar (0.5 Mpa, 70 psi) |
Sample preparation | 1. Adjust the sample to the composition of the start buffer, using one of these methods: Dilute the sample with start buffer. Exchange buffer using a HiPrep 26/10 Desalting, HiTrap Desalting or PD-10 Desalting column. 2. Filter the sample through a 0.45 µm filter or centrifuge at 10 000 × g for 10 min immediately before loading it to the column. This prevents clogging and increases the life time of the column when loading large sample volumes. |
Matrix | Highly cross-linked agarose |
Average particle size | 90 µm |
Ligand | Octylamine |
Dynamic binding capacity | ~22 mg ovalbumin/mL resin |
Chemical stability | Stable in commonly used aqueous buffers, 1 M NaOH, 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol. |
pH working range | 3 to 13 |
CIP stability | 3 to 14 |
Storage | 4°C to 30°C, 20% ethanol |
Cleaning-in-place | Wash with a solution of 1 M NaOH in 30% isopropanol or in 27% 1-propanol with reversed flow direction. |
Pack size | 5 × 1 mL |
Maximum flow velocity | 700 cm/h |
Column hardware pressure limit | 0.5 MPa (5 bar, 72 psi) |
Download Datasheet | Download SDS |
For Research or Industrial Raw Materials, Not For Personal Medical Use!
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