HiTrap Capto Core 700

• Cat#No#: Hi-288P
• Product Overview: HiTrap Capto Core 700 is prepacked with Capto Core 700, a multimodal BioProcess resin for intermediate purification and polishing of viruses and other large biomolecules.
• Description: Capto Core 700 is a novel, layered bead chromatography resin consisting of a porous outer layer and ligand-activated core. The octylamine ligands found in the core of the beads are multimodal, being both hydrophobic and positively charged. These internalized ligands bind impurities strongly over a wide range of pH and salt concentration. The pores of the bead outer layer prevent large targets (Mr > 700 000) from binding to the ligands while smaller impurities can enter freely into the beads where they are captured.
• Characteristic: High capacity and productivity in flowthrough mode.
  Prepacked HiTrap columns for easy screening and convenient process development, as well as small scale purification.
  Wide operational window of pH and conductivity.
• Maximum operating pressure: 5 bar (0.5 Mpa, 70 psi)
• Sample preparation: 1. Adjust the sample to the composition of the start buffer, using one of these methods: Dilute the sample with start buffer. Exchange buffer using a HiPrep 26/10 Desalting, HiTrap Desalting or PD-10 Desalting column.
  2. Filter the sample through a 0.45 µm filter or centrifuge at 10 000 × g for 10 min immediately before loading it to the column. This prevents clogging and increases the life time of the column when loading large sample volumes.
• Matrix: Highly cross-linked agarose
• Average particle size: 85 µm
• Ligand: Octylamine
• Dynamic binding capacity: ~13 mg ovalbumin/mL resin
• Recommended column height: 25 mm
• Chemical stability: Stable in commonly used aqueous buffers, 1 M NaOH, 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol.
• pH working range: 3 to 13
• CIP stability: 3 to 14
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: Wash with a solution of 1 M NaOH in 30% isopropanol or in 27% 1-propanol with reversed flow direction.
• Pack size: 5 × 1 mL
• Maximum flow velocity: 500 cm/h
• Dimensions: 7 × 25 mm
• Column volume: 1 mL
• Column i.d.: 7 mm
• Column hardware pressure limit: 0.5 MPa (5 bar, 72 psi)