HiTrap Capto DEAE

• Cat#No#: Hi-227P
• Product Overview: HiTrap Capto DEAE is a weak anion exchanger prepacked with BioProcess Capto resins for screening and small-scale protein purifications using ion exchange chromatography (IEX).
• Description: Capto DEAE has a weak diethylaminoethyl anion exchanger coupled to a chemically modified, high-flow agarose matrix. The modified agarose matrix provides greater particle rigidity without compromising pore size, which allows more flexibility in process-scale applications. High dynamic binding capacity is achieved with a dextran surface extender that coats the agarose matrix.
• Characteristic: High-throughput resin for capture and intermediate purification, excellent choice for process-scale purification.
  The combined properties of high binding capacity, high flow rate, and low back pressure reduces process cycle times and improves productivity at process scale.
  Rigid agarose matrix enables high bed heights and purification of viscous samples at high flow rates.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Metal ion capacity: 0.29 to 0.35 mmol Cl- /mL resin
• Matrix: Highly cross-linked agarose, spherical
• Ionic Exchanger Type: Weak anion, DEAE
• Average particle size: ~ 90 μm
• Dynamic binding capacity: > 90 mg ovalbumin/mL resin
• Recommended flow rate: 1 mL/min
• Recommended column height: 25 mm
• Chemical stability: Stable to commonly used aqueous buffers, 1.0 M NaOH, 8 M Urea, 6 M guanidine hydrochloride, 1 M acetic acid, 30% isopropanol, and 70% ethanol.
• pH working range: 2 to 12
• CIP stability: 2 to 14
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: 1. Wash with at least 2 column volumes of 2.0 M NaCl.
  2. Wash with at least 4 column volumes of 1.0 M NaOH.
  3. Wash with at least 2 column volumes of 2.0 M NaCl.
  4. Rinse with at least 2 column volumes of distilled water.
  5. Wash with 5 column volumes of start buffer for Capto Q and Capto S and at least 10 column volumes of start buffer for Capto DEAE or until eluent pH and conductivity have reached the required values.
• Pack size: 5 × 1 mL
• Maximum flow velocity: 700 cm/h
• Dimensions: 7 × 25 mm
• Column volume: 1 mL
• Column i.d.: 7 mm
• Column hardware pressure limit: 5 bar (0.5 MPa, 72 psi)
• Functional group: -N+H(CH2CH3)2