HiTrap Capto MMC ImpRes

• Cat#No#: Hi-290P
• Product Overview: HiTrap Capto MMC ImpRes is a ready-to-use column pre-packed with BioProcess Capto MMC ImpRes weak cation exchange multimodal resin for screening and small-scale protein purifications using ion exchange chromatography (IEX).
• Description: The multimodal functionality of Capto MMC ImpRes gives a different selectivity compared with traditional ion exchange columns and it binds proteins at high or low ionic strength.
• Characteristic: High yields achieved through the high-resolution beads and selectivity of the ligand.
  Enables use of a platform approach to MAb process development.
  Displays a broad pH/salt operational window.
  Columns for screening of selectivity, binding, and elution conditions, as well as for small scale purifications.
• Maximum operating pressure: 5 bar (0.5 Mpa, 70 psi)
• Sample preparation: 1. Adjust the sample to the composition of the start buffer, using one of these methods: Dilute the sample with start buffer. Exchange buffer using a HiPrep 26/10 Desalting, HiTrap Desalting or PD-10 Desalting column.
  2. Filter the sample through a 0.45 µm filter or centrifuge at 10 000 × g for 10 min immediately before loading it to the column. This prevents clogging and increases the life time of the column when loading large sample volumes.
• Matrix: Highly cross-linked agarose
• Ionic Exchanger Type: Multimodal weak cation exchanger
• Average particle size: 36 to 44 µm
• Ligand density: 25 to 39 µmol ligand/ml medium
• Recommended flow rate: 25 to 50 cm/h
• Recommended column height: 25 mm
• Chemical stability: All commonly used aqueous buffers, 1 M acetic acid, 1 M sodium hydroxide.
• pH working range: 3 to 12
• CIP stability: 2 to 14
• Storage: 4°C to 30°C in 20% ethanol, 0.2 M sodium acetate.
• Cleaning-in-place: 1. Wash with at least 2 column volumes (CV) of 2 M NaCl.
  2. Wash with at least 3 CV 1 M NaOH with at least 15 min contact time.
  3. Wash with at least 2 CV 2 M NaCl.
  4. Wash with at least 2 CV distilled water.
  5. Wash with 5 CV start buffer or until eluent pH and conductivity have reached the required values.
• Pack size: 5 × 1 mL
• Maximum flow velocity: 300 cm/h
• Dimensions: 7 × 25 mm
• Column volume: 1 mL
• Column i.d.: 7 mm
• Column hardware pressure limit: 5 bar (0.5 MPa)