HiTrap Capto SP ImpRes column

• Cat#No#: Hi-233P
• Product Overview: HiTrap Capto SP ImpRes chromatography column is packed with a strong cation exchange modern resin for high resolution, small-scale protein purification.
• Characteristic: Suitable for screening of selectivity, binding and elution conditions, and small-scale purifications.
  Sulfoethyl (S) strong cation exchanger.
  Optimized for capture and intermediate protein purification.
  Convenient HiTrap format for easy connection to a syringe, peristaltic pump, or chromatography system.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Sample preparation: 1. Dilution of cell paste: Add 5–10 ml of start buffer for each gram of cell paste.
  2. Enzymatic lysis: 0.2 mg/ml lysozyme, 20 μg/ml DNAse, 1 mM MgCl2, 1 mM Pefabloc SC or PMSF (final concentrations). Stir for 30 min at room temperature or 4°C depending on the sensitivity of the target protein.
  3. Mechanical lysis: Sonication on ice for approx. 10 min, homogenization with a French press or other homogenizer or freeze/thaw, repeated at least five times.
  4. Adjust the pH of the lysate. The pH should be at least 0.5 units below (cation exchangers) or 0.5 units above (anion exchangers) the pI of the target molecule. Do not use strong bases or acids for pH-adjustment (precipitation risk). Apply the unclarified lysate on the column directly after preparation.
• Metal ion capacity: 0.13 to 0.16 mmol (H+) /ml medium
• Matrix: High flow agarose
• Particle Size: 36 to 44 μm
• Dynamic binding capacity: >70 mg lysozyme /ml medium; >95 mg BSA /ml medium.
• Recommended flow rate: < 4 ml/min
• Recommended column height: 25 mm
• Chemical stability: All commonly used aqueous buffers, 1 M sodium hydroxide, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol and 70% ethanol.
• pH working range: 3 to 14
• CIP stability: 4 to 12
• Storage: 4 to 30°C, 0.2 M Sodium Acetate in 20% Ethanol.
• Cleaning-in-place: 1. Wash with at least 2 column volumes (CV) of 2 M NaCl.
  2. Wash with at least 4 CV of 1 M NaOH.
  3. Wash with at least 2 CV of 2 M NaCl.
  4. Rinse with at least 2 CV of distilled water.
  5. Wash with 5 CV of start buffer for Capto Q ImpRes and Capto SP ImpRes or until eluent pH and conductivity have reached the required values.
• Pack size: 5 × 1 mL
• Dimensions: 7 × 25 mm
• Column volume: 1 mL
• Column i.d.: 7 mm
• Column hardware pressure limit: 5 bar (0.5 MPa)
• Functional group: -CH2CH2CH2SO3 -