| Cat# | Product Name | Size | Price | Qty | Inquiry |
|---|---|---|---|---|---|
| Hi-073P | HiTrap Chelating HP columns | Inquiry |
| Product Information | |
| Cat#No# | Hi-073P |
| Product Overview | HiTrap Chelating HP columns are prepacked with an uncharged IMAC purification resin for purification of proteins and peptides with affinity for metal ions. |
| Description | HiTrap Chelating HP is one of a range of prepacked 1 ml and 5 ml columns for metal chelate affinity chromatography. Fast, simple, and easy separations are provided by the combination of the prepacked column and a high-performance affinity medium. HiTrap Chelating HP is particularly suitable for the isolation and purification of proteins and peptides containing exposed histidine residues. |
| Characteristic | Ready to charge with the metal ion of choice for optimized selectivity. Packed with Chelating Sepharose High Performance IMAC resin. Convenient affinity purification of native and recombinant proteins, including proteins with a histidine-tag (his-tag). Convenient prepacked columns provide fast, simple, and reproducible his-tag protein purification. HiTrap format compatible with a syringe, pump or chromatography system such as ÄKTA system or other FPLC system. |
| Maximum operating pressure | 5 bar [0.5 MPa] (70 psi) |
| Sample preparation | The sample should be adjusted to the composition of the binding buffer. This can be done by either diluting the sample with binding buffer or by buffer exchange using HiTrap Desalting, HiPrep 26/10 Desalting or PD-10 column. The sample should be filtered through a 0.45 μm filter or centrifuged immediately before it is applied to the column. |
| Metal ion capacity | ~ 23 μmol Cu2+/ml medium |
| Matrix | Highly cross-linked, spherical agarose |
| Average particle size | 34 µm |
| Ligand | Iminodiacetic acid |
| Dynamic binding capacity | Approx. 23 µmoles Cu2+/ml medium |
| Recommended flow rate | < 4 ml/min |
| Recommended column height | 25 mm |
| Chemical stability | Stable in all commonly used buffers and denaturants such as 6 M guanidine hydrochloride, 8 M urea and chaotropic salts. |
| pH working range | 4 to 12 |
| CIP stability | 3 to 13 |
| Temperature stability | 4°C to room temp |
| Storage | 4 to 30°C, 20% Ethanol |
| Pack size | 5 × 1 mL |
| Maximum flow velocity | 4 ml/min (1 ml); 20 ml/min (5 ml) |
| Maximum operating backpressure | 0.3 MPa, 3 bar |
| Dimensions | 7 × 25 mm |
| Column volume | 1 mL |
| Column i.d. | 7 mm |
| Column hardware pressure limit | 5 bar (70 psi, 0.5 MPa) |
Download Datasheet
|
|
For Research or Industrial Raw Materials, Not For Personal Medical Use!Online Inquiry
