| Cat# | Product Name | Size | Price | Qty | Inquiry |
|---|---|---|---|---|---|
| Hi-226P | HiTrap CM Sepharose FF | Inquiry |
| Product Information | |
| Cat#No# | Hi-226P |
| Product Overview | HiTrap CM FF is prepacked with CM Sepharose Fast Flow, and is a weak cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions. |
| Description | Q, SP, DEAE adn CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a resin with higher porosity, which is particularly useful for the purification of high molecular mass proteins. |
| Characteristic | Convenient and affordable for fast, easy ion exchange separations either alone or connected in series. The industry standard for ion exchange chromatography. High flow rates and good scale-up potential. Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient. Predictable scale-up. |
| Applications | For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules. |
| Maximum operating pressure | 5 bar [0.5 MPa] (70 psi) |
| Sample preparation | The sample must be adjusted to the composition of the start buffer by buffer exchange using HiTrap Desalting, HiPrep 26/10 Desalting or PD-10 columns. The sample must be filtered through a 0.45 μm filter or centrifuged immediately before it is applied to the column. |
| Metal ion capacity | 0.09–0.13 mmol H+/ mL resin |
| Matrix | Cross-linked agarose, 6%, spherical |
| Ionic Exchanger Type | Weak cation |
| Particle Size | 45–165 μm |
| Average particle size | ~ 90 μm |
| Dynamic binding capacity | ~ 50 mg Ribonuclease A/ mL resin. |
| Recommended flow rate | < 4 ml/min |
| Recommended column height | 25 mm |
| Chemical stability | Stable to commonly used aqueous buffers, 1.0 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 70% ethanol. |
| pH working range | 4 to 13 |
| CIP stability | 2 to 14 |
| Storage | 4 to 30°C, 20% Ethanol |
| Pack size | 5 × 1 mL |
| Dimensions | 7 × 25 mm |
| Column volume | 1 mL |
| Column i.d. | 7 mm |
| Column hardware pressure limit | 5 bar (0.5 MPa) |
| Functional group | -O-CH2COO |
Download Datasheet
|
|
For Research or Industrial Raw Materials, Not For Personal Medical Use!Online Inquiry
