HiTrap CM Sepharose FF

• Cat#No#: Hi-226P
• Product Overview: HiTrap CM FF is prepacked with CM Sepharose Fast Flow, and is a weak cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.
• Description: Q, SP, DEAE adn CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a resin with higher porosity, which is particularly useful for the purification of high molecular mass proteins.
• Characteristic: Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
  The industry standard for ion exchange chromatography.
  High flow rates and good scale-up potential.
  Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
  Predictable scale-up.
• Applications: For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Sample preparation: The sample must be adjusted to the composition of the start buffer by buffer exchange using HiTrap Desalting, HiPrep 26/10 Desalting or PD-10 columns. The sample must be filtered through a 0.45 μm filter or centrifuged immediately before it is applied to the column.
• Metal ion capacity: 0.09–0.13 mmol H+/ mL resin
• Matrix: Cross-linked agarose, 6%, spherical
• Ionic Exchanger Type: Weak cation
• Particle Size: 45–165 μm
• Average particle size: ~ 90 μm
• Dynamic binding capacity: ~ 50 mg Ribonuclease A/ mL resin.
• Recommended flow rate: < 4 ml/min
• Recommended column height: 25 mm
• Chemical stability: Stable to commonly used aqueous buffers, 1.0 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 70% ethanol.
• pH working range: 4 to 13
• CIP stability: 2 to 14
• Storage: 4 to 30°C, 20% Ethanol
• Pack size: 5 × 1 mL
• Dimensions: 7 × 25 mm
• Column volume: 1 mL
• Column i.d.: 7 mm
• Column hardware pressure limit: 5 bar (0.5 MPa)
• Functional group: -O-CH2COO