HiTrap Con A 4B Columns

• Cat#No#: Hi-074P
• Product Overview: HiTrap Con A 4B is a ready-to-use column prepacked with Con A Sepharose 4B, a resin for convenient separation and purification of glycoproteins, polysaccharides, and glycolipids.
• Description: Concanavalin A (Con A) is a tetrameric metalloprotein isolated from Canavalia ensiformis (jack bean). Con A binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl, and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose is routinely used for separation and purification of glycoproteins, polysaccharides, and glycolipids.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Matrix: 4% agarose
• Average particle size: ~ 90 μm
• Ligand: Concanavalin A
• Ligand density: 10 to 15 mg Con A/mL resin
• Dynamic binding capacity: 20 to 45 mg porcine thyroglobulin/mL resin.
• Recommended flow rate: 0.1 to 1 mL/min (1 mL); 0.5 to 5 mL/min (5 mL).
• Recommended column height: 25 mm
• Chemical stability: Stable to all commonly used aqueous buffers. Chelating agents such as EDTA, 8 M urea, or solutions having a pH below 3 should be avoided as these conditions results in removal of manganese from the lectin with loss of activity as a result.
• pH working range: 4 to 9
• Storage: 4 to 8°C, 20% Ethanol containing 0.1 M Acetate Buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl2.
• Pack size: 5 × 1 mL
• Maximum flow velocity: 1 mL/min (1 mL); 5 mL/min (5 mL).
• Dimensions: 7 × 25 mm
• Column volume: 1 mL
• Column i.d.: 7 mm
• Column hardware pressure limit: 0.5 MPa (5 bar)