HiTrap Heparin HP columns

• Cat#No#: Hi-075P
• Product Overview: HiTrap Heparin HP columns are designed for high-resolution purification of DNA-binding proteins, coagulation factors, and other plasma proteins.
• Description: HiTrap Heparin HP is one of a range of prepacked, ready to use columns for affinity chromatography. Fast, simple and easy separations are provided by the prepacked column with a high performance affinity medium. HiTrap Heparin HP is particulary suitable for the isolation and purification of antithrombin III and other coagulation factors, lipoproteins, lipases, protein synthesis factors, hormones, steroid receptors, nucleic acid binding enzymes and interferon.
• Characteristic: Suitable for purification of antithrombin III, coagulation factors, and other plasma proteins.
  Useful for screening and optimization before scaling up to bulk resins such as Heparin 6 Fast Flow.
  High-resolution purification and elution in narrow peaks, minimizing the need for further concentration steps.
  Prepacked with Heparin Sepharose High Performance resin.
  HiTrap format compatible with syringes, pumps, and chromatography systems such as ÄKTA systems.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Sample preparation: The sample should be adjusted to the composition of the binding buffer. This can be done by either diluting the sample with binding buffer or by buffer exchange using HiTrap Desalting, HiPrep 26/10 Desalting or PD-10 column. The sample should be filtered through a 0.45 μm filter or centrifuged immediately before it is applied to the column.
• Matrix: Highly cross-linked 6% spherical agarose
• Average particle size: 34 μm
• Ligand: Heparin
• Ligand density: approx. 10 mg/ml
• Dynamic binding capacity: approx. 3 mg antithrombin III/ml medium
• Recommended flow rate: < 4 ml/min
• Recommended column height: 25 mm
• Chemical stability: All commonly used buffers.
• pH working range: 5 to 10
• CIP stability: 5 to 10
• Temperature stability: 4°C to room temp.
• Storage: 4 to 30°C, 20% Ethanol
• Pack size: 5 × 1 mL
• Maximum flow velocity: 4 ml/min (1 ml), 20 ml/min (5 ml)
• Dimensions: 7 × 25 mm
• Column volume: 1 mL
• Column i.d.: 7 mm
• Column hardware pressure limit: 5 bar (0.5 MPa, 70 psi)