HiTrap IXSelect

• Cat#No#: Hi-078P
• Product Overview: HiTrap IXSelect columns are prepacked with IXSelect resin for screening and small-scale protein purifications using affinity chromatography.
• Description: IXSelect is an affinity chromatography resin designed for selective binding of coagulation Factor IX (FIX). The resin can be used, under mild elution conditions, for purifying FIX from plasma as well as from recombinant sources. IXSelect is part of Cytiva's Custom Designed Media program.
• Characteristic: Efficient, industrial-scale purification of FIX by affinity chromatography.
  High flow rates for processing of large sample volumes to increase throughput.
  Reduced regulatory concerns (due to non-mammalian derived product) in the production of FIX for clinical applications.
• Matrix: Highly cross-linked agarose, spherical
• Average particle size: 75 μm
• Ligand: Recombinant protein (Mr 13 151) produced in Saccharomyces cerevisiae.
• Ligand density: Approx. 8 mg/mL resin
• Dynamic binding capacity: Approx. 6 mg FIX/mL resin
• Recommended flow rate: Minimum 600 cm/h in a 1 m diameter column, with 20 cm bed height at 20˚C using buffers with the same viscosity as water at < 0.3 MPa (3 bar)
• pH working range: 3 to 10
• CIP stability: 2 to 12
• Storage: 2–8°C
• Pack size: 5 × 1 mL
• Wash buffer: 20 mM Tris-HCl, 500 mM NaCl, 0.01% Tween 80, pH 7.4.