HiTrap LambdaFabSelect

• Cat#No#: Hi-015P
• Product Overview: LambdaFabSelect is an affinity chromatography resin, designed for the purification of lambda Fab fragments, with high binding capacity, purity, and yield.
• Description: Antibody fragments are gaining increased attention as potential biopharmaceuticals because they display certain advantages over monoclonal antibodies (MAbs). For example, Fabs show improved pharmacokinetics for tissue penetration and can bind to targets inaccessible to conventional antigen binding sites.
  LambdaFabSelect are affinity chromatography resins for purifying kappa and lambda Fab fragments, respectively. These resins enable efficient capture with high purity and yield.
• Characteristic: Efficient, industrial-scale capture of Fabs by affinity chromatography.
  High binding capacity for Fabs.
  Rigid agarose base matrix allows high flow rates and processing of large sample volumes for increased throughput.
  Non-mammalian derived product reduces regulatory concerns in the production of Fabs for clinical applications.
  Low ligand leakage ensures increased Fab purity and productivity.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Matrix: Highly cross-linked agarose, spherical
• Average particle size: 75 μm
• Ligand: Recombinant protein (Mr 13 000), produced in Saccharomyces cerevisiae, that binds to the constant region of Fab kappa or lambda light chain.
• Ligand density: Approx. 7 mg/mL resin.
• Dynamic binding capacity: Approx. 20 mg Fab/mL resin.
• Recommended flow rate: At least 600 cm/h in a 1 m diameter column, with 20 cm bed height at 20°C using buffers with the same viscosity as water at < 0.3 MPa (3 bar).
• Recommended column height: 25 mm
• pH working range: 3 to 10
• CIP stability: 2 to 12
• Temperature stability: 4°C to 30°C
• Storage: 4 to 8°C, 20% Ethanol
• Pack size: 5 × 1 mL