| Cat# | Product Name | Size | Price | Qty | Inquiry |
|---|---|---|---|---|---|
| Hi-016P | HiTrap MabSelect | Inquiry |
| Product Information | |
| Cat#No# | Hi-016P |
| Product Overview | HiTrap MabSelect columns are prepacked with MabSelect, a BioProcess resin for capturing of mAbs from large sample volumes. |
| Characteristic | Increased binding capacity compared with other protein A resins. High purity in one step. Convenient, prepacked 1 mL and 5 mL columns. Excellent for process development and screening of purification conditions. Simple operation with a syringe, a pump, an ÄKTA system, or other chromatography systems. |
| Maximum operating pressure | 5 bar [0.5 MPa] (70 psi) |
| Matrix | Highly cross-linked agarose, spherical |
| Average particle size | ~ 85 µm |
| Ligand | Recombinant protein A (E. coli). |
| Coupling chemistry | Epoxy |
| Dynamic binding capacity | ~ 30 mg hIgG/mL resin |
| Recommended flow rate | < 4 ml/min |
| Recommended column height | 25 mm |
| Chemical stability | Stable to commonly used aqueous buffers, 10 mM NaOH (pH 12), 0.1 M sodium citrate/HCl (pH 3), 6M guanidine HCl, 8M urea, 20% ethanol, 2% benzyl alcohol. |
| pH working range | 3 to 10 |
| CIP stability | 3.0 to 12.4 |
| Temperature stability | 2°C to 40°C |
| Storage | 20% ethanol, 2°C to 8°C |
| Shipping | 20% ethanol |
| Cleaning-in-place | 1. Wash with 2 column volumes of 10 mM NaOH, contact time approx. 30 min. 2. Wash immediately with at least 5 column volumes of filtered binding buffer at pH 7 to 8. or 1. Wash with 2 column volumes of 6 M guanidine hydrochloride, contact time approx. 10 min. 2. Wash immediately with at least 5 column volumes of filtered binding buffer at pH 7 to 8. |
| Sanitization | 1. Equilibrate the column with 0.1 M acetic acid in 20% ethanol. 2. Allow to stand for 1 hour, and wash with at least 5 column volumes of sterile binding buffer. or 1. Equilibrate the column with 70% ethanol. 2. Allow to stand for 12 hours, and wash with at least 5 column volumes of sterile binding buffer. |
| Pack size | 5 × 1 mL |
| Maximum flow velocity | 4 mL/min for 1 mL and 20 mL/min for 5 mL column. |
| Dimensions | 7 × 25 mm |
| Column volume | 1 ml |
| Column i.d. | 7 mm |
| Column hardware pressure limit | 5 bar (0.5 MPa, 70 psi) |
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For Research or Industrial Raw Materials, Not For Personal Medical Use!Online Inquiry
