HiTrap MabSelect

• Cat#No#: Hi-016P
• Product Overview: HiTrap MabSelect columns are prepacked with MabSelect, a BioProcess resin for capturing of mAbs from large sample volumes.
• Characteristic: Increased binding capacity compared with other protein A resins.
  High purity in one step.
  Convenient, prepacked 1 mL and 5 mL columns.
  Excellent for process development and screening of purification conditions.
  Simple operation with a syringe, a pump, an ÄKTA system, or other chromatography systems.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Matrix: Highly cross-linked agarose, spherical
• Average particle size: ~ 85 µm
• Ligand: Recombinant protein A (E. coli).
• Coupling chemistry: Epoxy
• Dynamic binding capacity: ~ 30 mg hIgG/mL resin
• Recommended flow rate: < 4 ml/min
• Recommended column height: 25 mm
• Chemical stability: Stable to commonly used aqueous buffers, 10 mM NaOH (pH 12), 0.1 M sodium citrate/HCl (pH 3), 6M guanidine HCl, 8M urea, 20% ethanol, 2% benzyl alcohol.
• pH working range: 3 to 10
• CIP stability: 3.0 to 12.4
• Temperature stability: 2°C to 40°C
• Storage: 20% ethanol, 2°C to 8°C
• Shipping: 20% ethanol
• Cleaning-in-place: 1. Wash with 2 column volumes of 10 mM NaOH, contact time approx. 30 min. 2. Wash immediately with at least 5 column volumes of filtered binding buffer at pH 7 to 8. or 1. Wash with 2 column volumes of 6 M guanidine hydrochloride, contact time approx. 10 min. 2. Wash immediately with at least 5 column volumes of filtered binding buffer at pH 7 to 8.
• Sanitization: 1. Equilibrate the column with 0.1 M acetic acid in 20% ethanol. 2. Allow to stand for 1 hour, and wash with at least 5 column volumes of sterile binding buffer. or 1. Equilibrate the column with 70% ethanol. 2. Allow to stand for 12 hours, and wash with at least 5 column volumes of sterile binding buffer.
• Pack size: 5 × 1 mL
• Maximum flow velocity: 4 mL/min for 1 mL and 20 mL/min for 5 mL column.
• Dimensions: 7 × 25 mm
• Column volume: 1 ml
• Column i.d.: 7 mm
• Column hardware pressure limit: 5 bar (0.5 MPa, 70 psi)