HiTrap MabSelect PrismA
Home / Products / Chromatography Reagents / Antibody affinity chromatography /

HiTrap MabSelect PrismA

Cat# Product Name Size Price Qty Inquiry
Hi-017P HiTrap MabSelect PrismA Inquiry
Product Information
Cat#No# Hi-017P
Product Overview These HiTrap columns are prepacked with MabSelect PrismA protein A chromatography resin. This affinity resin in the packed column has been improved with an optimized high-flow agarose base matrix and a genetically engineered protein ligand, allowing efficient cleaning between monoclonal antibody purification runs. This allows future demands in monoclonal antibody purification to be met, including processing of many bispecific antibodies.
Characteristic Enhanced dynamic binding capacity compared with other protein A resins.
Excellent alkaline stability enables efficient cleaning and sanitization using 0.5–1.0 M NaOH.
Convenient HiTrap format for easy connection to a syringe, peristaltic pump, or chromatography systems such as an ÄKTA system for convenient process optimization.
Maximum operating pressure 5 bar (0.5 MPa, 70 psi)
Matrix Rigid, highly cross-linked agarose
Average particle size ~ 60 μm
Dynamic binding capacity ~ 40 mg polyclonal IgG/mL resin, 2 minutes residence time; ~ 80 mg polyclonal IgG/mL resin, 6 minutes residence time.
Recommended flow rate < 4 mL/min
Recommended column height 25 mm
Chemical stability Stable to commonly used aqueous buffers for Protein A chromatography.
pH working range 3 to 12
CIP stability 2 to 14
Temperature stability 2°C to 40°C
Storage 20% ethanol, 2°C to 8°C
Cleaning-in-place 1. Wash the column with 3 column volumes (CV) of binding buffer.
2. Wash with at least 3 CV 0.5 to 1.0 M NaOH with a contact time of 15 minutes.
3. Wash immediately with at least 5 CV sterile and filtered binding buffer at pH 7 to 8.
Sanitization 1. Wash the column with 3 column volumes (CV) of binding buffer.
2. Wash the column with at least 3 CV 0.5 to 1.0 M NaOH.
3. Use a contact time of at least 15 minutes for 0.5 to 1.0 M NaOH (see also the note below).
4. Wash immediately with at least 5 CV sterile and filtered binding buffer at pH 7 to 8.
Pack size 1 × 1 mL
Maximum flow velocity 4 mL/min
Dimensions 7 × 25 mm
Column volume 1 mL
Column i.d. 7 mm
Download Datasheet
For Research or Industrial Raw Materials, Not For Personal Medical Use!
Online Inquiry
Copyright © Creative BioMart. All rights reserved.
0
Inquiry Basket