HiTrap MabSelect PrismA

• Cat#No#: Hi-017P
• Product Overview: These HiTrap columns are prepacked with MabSelect PrismA protein A chromatography resin. This affinity resin in the packed column has been improved with an optimized high-flow agarose base matrix and a genetically engineered protein ligand, allowing efficient cleaning between monoclonal antibody purification runs. This allows future demands in monoclonal antibody purification to be met, including processing of many bispecific antibodies.
• Characteristic: Enhanced dynamic binding capacity compared with other protein A resins.
  Excellent alkaline stability enables efficient cleaning and sanitization using 0.5–1.0 M NaOH.
  Convenient HiTrap format for easy connection to a syringe, peristaltic pump, or chromatography systems such as an ÄKTA system for convenient process optimization.
• Maximum operating pressure: 5 bar (0.5 MPa, 70 psi)
• Matrix: Rigid, highly cross-linked agarose
• Average particle size: ~ 60 μm
• Dynamic binding capacity: ~ 40 mg polyclonal IgG/mL resin, 2 minutes residence time; ~ 80 mg polyclonal IgG/mL resin, 6 minutes residence time.
• Recommended flow rate: < 4 mL/min
• Recommended column height: 25 mm
• Chemical stability: Stable to commonly used aqueous buffers for Protein A chromatography.
• pH working range: 3 to 12
• CIP stability: 2 to 14
• Temperature stability: 2°C to 40°C
• Storage: 20% ethanol, 2°C to 8°C
• Cleaning-in-place: 1. Wash the column with 3 column volumes (CV) of binding buffer.
  2. Wash with at least 3 CV 0.5 to 1.0 M NaOH with a contact time of 15 minutes.
  3. Wash immediately with at least 5 CV sterile and filtered binding buffer at pH 7 to 8.
• Sanitization: 1. Wash the column with 3 column volumes (CV) of binding buffer.
  2. Wash the column with at least 3 CV 0.5 to 1.0 M NaOH.
  3. Use a contact time of at least 15 minutes for 0.5 to 1.0 M NaOH (see also the note below).
  4. Wash immediately with at least 5 CV sterile and filtered binding buffer at pH 7 to 8.
• Pack size: 1 × 1 mL
• Maximum flow velocity: 4 mL/min
• Dimensions: 7 × 25 mm
• Column volume: 1 mL
• Column i.d.: 7 mm