Cat# | Product Name | Size | Price | Qty | Inquiry |
---|---|---|---|---|---|
Hi-017P | HiTrap MabSelect PrismA | Inquiry |
Product Information | |
Cat#No# | Hi-017P |
Product Overview | These HiTrap columns are prepacked with MabSelect PrismA protein A chromatography resin. This affinity resin in the packed column has been improved with an optimized high-flow agarose base matrix and a genetically engineered protein ligand, allowing efficient cleaning between monoclonal antibody purification runs. This allows future demands in monoclonal antibody purification to be met, including processing of many bispecific antibodies. |
Characteristic | Enhanced dynamic binding capacity compared with other protein A resins. Excellent alkaline stability enables efficient cleaning and sanitization using 0.5–1.0 M NaOH. Convenient HiTrap format for easy connection to a syringe, peristaltic pump, or chromatography systems such as an ÄKTA system for convenient process optimization. |
Maximum operating pressure | 5 bar (0.5 MPa, 70 psi) |
Matrix | Rigid, highly cross-linked agarose |
Average particle size | ~ 60 μm |
Dynamic binding capacity | ~ 40 mg polyclonal IgG/mL resin, 2 minutes residence time; ~ 80 mg polyclonal IgG/mL resin, 6 minutes residence time. |
Recommended flow rate | < 4 mL/min |
Recommended column height | 25 mm |
Chemical stability | Stable to commonly used aqueous buffers for Protein A chromatography. |
pH working range | 3 to 12 |
CIP stability | 2 to 14 |
Temperature stability | 2°C to 40°C |
Storage | 20% ethanol, 2°C to 8°C |
Cleaning-in-place | 1. Wash the column with 3 column volumes (CV) of binding buffer. 2. Wash with at least 3 CV 0.5 to 1.0 M NaOH with a contact time of 15 minutes. 3. Wash immediately with at least 5 CV sterile and filtered binding buffer at pH 7 to 8. |
Sanitization | 1. Wash the column with 3 column volumes (CV) of binding buffer. 2. Wash the column with at least 3 CV 0.5 to 1.0 M NaOH. 3. Use a contact time of at least 15 minutes for 0.5 to 1.0 M NaOH (see also the note below). 4. Wash immediately with at least 5 CV sterile and filtered binding buffer at pH 7 to 8. |
Pack size | 1 × 1 mL |
Maximum flow velocity | 4 mL/min |
Dimensions | 7 × 25 mm |
Column volume | 1 mL |
Column i.d. | 7 mm |
Download Datasheet |
For Research or Industrial Raw Materials, Not For Personal Medical Use!
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