HiTrap MabSelect SuRe

• Cat#No#: Hi-018P
• Product Overview: HiTrap MabSelect SuRe columns are prepacked with MabSelect SuRe, a BioProcess resin for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allow cleaning-in-place (CIP).
• Characteristic: Withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
  Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage.
  Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
  Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
  Prepacked HiTrap columns for process development, screening of purification conditions, and small-scale purification of Mabs.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Matrix: Highly cross-linked agarose, spherical
• Average particle size: ~ 85 μm
• Ligand: Alkali-tolerant, protein A (E. coli)
• Coupling chemistry: Epoxy
• Dynamic binding capacity: ~ 35 mg IgG/mL resin
• Recommended flow rate: < 4 ml/min
• Recommended column height: 25 mm
• Chemical stability: Stable to commonly used aqueous buffers.
• pH working range: 3 to 12
• CIP stability: 3 to 13.7
• Temperature stability: 2°C to 40°C
• Cleaning-in-place: 1. Wash the column with 3 column volumes of binding buffer.
  2. Wash with at least 2 column volumes of 0.1 to 0.5 M NaOH. Contact time 10 to 15 minutes.
  3. Wash immediately with at least 5 column volumes of binding buffer.
• Sanitization: 1. Wash the column with 3 column volumes of binding buffer.
  2. Equilibrate the column with 0.1 to 0.5 M NaOH.
  3. Use a contact time of at least 15 minutes (see the note below).
  4. Wash immediately with at least 5 column volumes of binding buffer.
• Pack size: 5 × 1 mL
• Maximum flow velocity: 4 mL/min
• Dimensions: 7 × 25 mm
• Column volume: 1 mL
• Column i.d.: 7 mm
• Column hardware pressure limit: 0.5 MPa (5 bar)