| Product Information | |
| Cat#No# | Hi-023P |
| Product Overview | HiTrap chromatography columns with MabSelect VL protein L resin. A redesigned protein L ligand captures antibody Fabs, bispecifics, and other variants containing a kappa light chain with high binding capacity and improved alkaline stability. |
| Description | MabSelect VL affinity resin uses a protein L ligand with strong affinity for the variable region of a human antibody's kappa light chain. The resin offers high productivity and robust processes for affinity capture of bispecific antibodies and antibody fragments containing the kappa light chain and offers a good capture alternative for antibody variants that does not bind to protein A. This resin has substantially improved dynamic binding capacity (DBC) and alkaline stability compared to its predecessor, making it well suited for cost-efficient capture of antibody variants. MabSelect VL resin allows for good resolution of product-related impurities in the capture of bispecific antibodies, and it provides a tool for efficient purification of antibody variants to high purity. |
| Characteristic | High dynamic binding capacity for efficient capture of target molecules. Stable when cleaned with 0.1 M NaOH, reducing risk for bioburden incidents. Provides good resolution for product-related impurities in the capture of bispecific antibodies. |
| Maximum operating pressure | 5 bar [0.5 MPa] (70 psi) |
| Ligand Coupling Method | Single point attachment |
| Matrix | Highly cross-linked agarose, spherical |
| Average particle size | ~ 60 µm |
| Ligand | Alkaline stabilized protein L-derived (E. coli) |
| Coupling chemistry | Epoxy |
| Dynamic binding capacity | 70 mg human IgG/mL resin at 6 min residence time; ~ 60 mg human IgG /mL resin at 4 min residence time |
| Recommended flow rate | < 4 mL/min |
| Recommended column height | 25 mm |
| Chemical stability | Stable in aqueous buffers commonly used in protein L chromatography. |
| pH working range | 2 to 10 |
| CIP stability | 2 to 13 (recommended 0.1 M NaOH) |
| Shipping | 20% ethanol or 2% benzyl alcohol (BnOH). |
| Pack size | 1 × 1 mL |
| Maximum flow velocity | 300 cm/h |
| Dimensions | 7 × 25 mm |
| Column volume | 1 mL |
| Column i.d. | 7 mm |
| Column hardware pressure limit | 5 bar (0.5 MPa) |
Download Datasheet |
|
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.Online Inquiry