HiTrap MabSelect Xtra

• Cat#No#: Hi-020P
• Product Overview: HiTrap MabSelect Xtra columns are prepacked with MabSelect Xtra, a BioProcess resin for capture of monoclonal antbodies from large sample volumes. The increased capacity of the resin is well suited for purification of Mabs from high-level expression feedstock.
• Description: HiTrap MabSelect Xtra is members of the HiTrap family of prepacked columns for purification of monoclonal antibodies (mAbs).
• Characteristic: Increased dynamic binding capacity with smaller particle size and greater porosity than MabSelect resin.
  MabSelect Xtra uses the same recombinant protein A ligand as MabSelect Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
  Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
  Prepacked HiTrap columns for process development, screening of purification conditions, and small-scale purification of Mabs.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Matrix: Highly cross-linked agarose, spherical
• Average particle size: ~ 75 µm
• Ligand: Recombinant protein A (E. coli)
• Coupling chemistry: Epoxy
• Dynamic binding capacity: ~ 40 mg hIgG/mL resin
• Recommended flow rate: < 4 ml/min
• Recommended column height: 25 mm
• Chemical stability: Stable to commonly used aqueous buffers, 10 mM NaOH (pH 12), 0.1 M sodium citrate/HCl (pH 3), 6M guanidine HCl, 20% ethanol, 2% benzyl alcohol.
• pH working range: 3 to 10
• CIP stability: 3.0 to 12.4
• Temperature stability: 2°C to 40°C
• Storage: 20% ethanol, 2˚C to 8˚C
• Shipping: 20% ethanol
• Cleaning-in-place: 1. Wash the column with 2 column volumes of a nonionic detergent (e.g., conc. 0.1%), contact time approx. 10 min. 2. Wash immediately with at least 5 column volumes of filtered binding buffer at pH 7 to 8. or 1. Wash the column with 3 to 4 column volumes of 70% ethanol, contact time approx. 10 min. 2. Wash immediately with at least 5 column volumes of filtered binding buffer at pH 7 to 8. Apply increasing gradients to avoid air bubble formation when using high concentrations of organic solvents. or 1. Wash the column with 3 to 4 column volumes of 30% isopropanol, contact time approx. 10 min. 2. Wash immediately with at least 5 column volumes of filtered binding buffer at pH 7 to 8. Apply increasing gradients to avoid air bubble formation when using high concentrations of organic solvents.
• Sanitization: 1. Equilibrate the column with 0.1 M acetic acid in 20% ethanol. 2. Allow to stand for 1 hour, and wash with at least 5 column volumes of sterile binding buffer. or 1. Equilibrate the column with 70% ethanol. 2. Allow to stand for 12 hours, and wash with at least 5 column volumes of sterile binding buffer.
• Pack size: 5 × 1 mL
• Maximum flow velocity: 4 mL/min for 1 mL and 20 mL/min for 5 mL column
• Dimensions: 7 × 25 mm
• Column volume: 1 mL
• Column i.d.: 7 mm
• Column hardware pressure limit: 5 bar (0.5 MPa, 70 psi)