HiTrap NHS-Activated HP columns

• Cat#No#: Hi-079P
• Product Overview: HiTrap NHS-activated HP prepacked chromatography columns allow you to design an affinity column suitable for your purification needs.
• Description: HiTrap is a range of prepacked columns for preparative affinity chromatography. Fast, simple, and easy separations are provided by the combination of a specially designed column and a high-performance affinity medium. HiTrap NHS-activated HP is the pre-activated member of this column family. The coupling method supplied is easy to perform; normally the affinity medium is ready to use in less than an hour. HiTrap NHS-activated HP is available in 1 ml and 5 ml prepacked columns.
• Characteristic: Easy coupling of any ligand containing primary amine groups.
  Packed with NHS-Activated Sepharose High Performance resin.
  Convenient prepacked columns can be used with a syringe, pump, or chromatographic system, such as ÄKTA systems.
• Applications: Partial purification of an IgE-stimulating factor from a human T-cell line.
  Removal of BSA from an HIV-2 virus lysate.
  Purification of anti-mouse Fc IgG from sheep anti-serum.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Matrix: Highly cross-linked, spherical agarose
• Average particle size: 34 µm
• Ligand: NHS groups
• Ligand density: 10 µmol/ml medium
• Coupling chemistry: pH 6.5 to 9, room temperature, 15–30 min or pH 6.5 to 9, 4°C, 4 hours.
• Recommended flow rate: < 4 ml/min
• Recommended column height: 25 mm
• Chemical stability: All common used buffers
• pH working range: 3–12
• CIP stability: 3–12
• Temperature stability: 4°C to room temperature
• Storage: 4 to 8°C, 100% Isopropanol
• Pack size: 5 × 1 mL
• Maximum flow velocity: 4 ml/min (1 ml); 20 ml/min (5 ml).
• Dimensions: 7 × 25 mm
• Column volume: 1 mL
• Column i.d.: 7 mm
• Column hardware pressure limit: 5 bar (0.5 MPa)