| Product Information | |
| Cat#No# | Hi-158P |
| Product Overview | HiTrap Phenyl FF (high sub) are prepacked Phenyl Sepharose 6 Fast Flow columns for hydrophobic interaction chromatography (HIC). The columns are used for small-scale protein purification as well as for screening of binding and elutions conditions. |
| Characteristic | Provide convenient and fast small-scale protein purifications. Phenyl Sepharose 6 Fast Flow is a well-established, standard aromatic HIC resin for capture and intermediate purification requiring low to resin hydrophobicity. Two levels of substitution on Phenyl Sepahrose 6 Fast Flow help find optimal selectivity and binding capacity. Flexible design for use with syringes, single laboratory pump, or chromatography system. High flow rates and high capacities. Excellent and easy scale-up achieved by connecting several HiTrap columns in series or using HiPrep columns. |
| Applications | For screening of selectivity, binding and elution conditions, as well as small scale purifications. |
| Maximum operating pressure | 5 bar [0.5 MPa] (70 psi) |
| Recommended flow rate | < 1 ml/min |
| Recommended column height | 25 mm |
| Storage | 4°C to 30°C, 20% Ethanol |
| Pack size | 5 × 1 mL |
| Maximum operating backpressure | 4 mL/min |
| Dimensions | 7 × 25 mm |
| Column i.d. | 7 mm |
| Column hardware pressure limit | 5 bar (0.5 MPa) |
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