HiTrap Protein A HP columns

• Cat#No#: Hi-021P
• Product Overview: HiTrap Protein A HP are prepacked columns for routine preparative purification of monoclonal and polyclonal antibodies such as human IgG.
• Characteristic: First choice Protein A resin for routine purification of antibodies: ensures minimized sample dilution and high resolution.
  Small bead size (34 µm) ensures narrow elution peaks containing concentrated material.
• Applications: Can be operated with a syringe, peristaltic pump or liquid chromatography system such as ÄKTA design.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Sample preparation: The sample should be adjusted to the composition of the binding buffer. This can be done by either diluting the sample with binding buffer or by buffer exchange using HiTrap Desalting, PD-10 Desalting or HiPrep 26/10 Desalting columns. The sample should be filtered through a 0.45 μm filter or centrifuged immediately before it is applied to the column.
• Matrix: Highly cross-linked spherical agarose
• Average particle size: 34 μm
• Ligand: Protein A, Mr ~42 000
• Dynamic binding capacity: ~20 mg human IgG/ml medium
• Recommended flow rate: < 4 ml/min
• Recommended column height: 25 mm
• Chemical stability: All commonly used buffers
• pH working range: 3 to 9
• CIP stability: 2 to 9
• Storage: 4 to 8°C, 20% Ethanol
• Pack size: 5 × 1 mL
• Maximum flow velocity: 4 and 20 ml/min for 1 ml and 5 ml column respectively
• Maximum operating backpressure: 0.3 MPa, 3 bar
• Dimensions: 7 × 25 mm
• Column volume: 1 mL
• Column i.d.: 7 mm
• Column hardware pressure limit: 5 bar (0.5 MPa)