HiTrap Protein G HP columns

• Cat#No#: Hi-080P
• Product Overview: HiTrap Protein G HP are prepacked columns for general-purpose preparative purification of monoclonal and polyclonal antibodies from most species including rat.
• Characteristic: First choice Protein G resin for routine purification of antibodies: ensures minimized sample dilution and high resolution.
  The small bead size (34 μm) ensures narrow elution peaks containing concentrated material.
  High protein recovery due to high binding capacity of 25 mg IgG/mL resin.
  Broader selectivity compared with protein A ligand.
  Convenient HiTrap format for easy connection to a syringe, peristaltic pump, or chromatography system such as ÄKTA system.
• Applications: Designed for use with a syringe, peristaltic pump, or chromatography system.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Sample preparation: The sample should be adjusted to the composition of the binding buffer. This can be done by either diluting the sample with binding buffer or by buffer exchange using HiTrap Desalting, HiPrep 26/10 Desalting or PD-10 Desalting columns.
  The sample should be fully solubilized. We recommend centifugation or filtration immediately before loading on the column to remove particulate material (0.45 μm filter).
• Matrix: Highly cross-linked spherical agarose
• Average particle size: 34 μm
• Ligand: Recombinant protein A (E. coli), protein A or protein G
• Dynamic binding capacity: 25 mg human IgG/column
• Recommended flow rate: < 4 ml/min
• Recommended column height: 25 mm
• Chemical stability: All commonly used buffers.
• pH working range: 3 to 9
• CIP stability: 2 to 9
• Storage: 2 to 8°C, 20% Ethanol
• Pack size: 5 × 1 mL
• Maximum flow velocity: 4 and 20 mL/min for 1 and 5 mL columns, respectively.
• Dimensions: 7 × 25 mm
• Column volume: 1 mL
• Column hardware pressure limit: 0.5 MPa (5 bar, 70 psi)