HiTrap Q FF column

• Cat#No#: Hi-236P
• Product Overview: HiTrap Q FF is a strong anion exchange chromatography column for small-scale protein purification.
• Characteristic: Packed with Q Sepharose Fast Flow strong anion exchange resin.
  Convenient and affordable for fast ion exchange separations, either alone or connected in series.
  High flow rates and good scale-up potential.
  Resin made up of 6% crosslinked agarose and 90 µm particle size.
  pH stable: 2–12 operational, 2–14 for cleaning.
  Convenient HiTrap format for easy connection to a syringe, peristaltic pump, or chromatography system.
• Applications: For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Sample preparation: The sample should be adjusted to the composition of the start buffer by buffer exchange using HiTrap Desalting, HiPrep 26/10 Desalting or PD-10 columns, see Table 5. The sample should be filtered through a 0.45 μm filter or centrifuged immediately before it is applied to the column.
• Metal ion capacity: 0.18–0.24 mmol Cl- /mL resin
• Matrix: Cross-linked agarose, 6%, spherical
• Ionic Exchanger Type: Strong anion
• Average particle size: ~ 90 μm
• Dynamic binding capacity: ~ 120 mg HSA/ mL resin
• Recommended flow rate: < 4 ml/min
• Recommended column height: 25 mm
• Chemical stability: Stable to commonly used aqueous buffers, 1.0 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 70% ethanol.
• pH working range: 2 to 12
• CIP stability: 2 to 14
• Storage: 4 to 30°C, 20% Ethanol
• Pack size: 5 × 1 mL
• Dimensions: 7 × 25 mm
• Column volume: 1 mL
• Column i.d.: 7 mm
• Column hardware pressure limit: 5 bar (0.5 MPa)
• Functional group: -N+(CH3)3