HiTrap rProtein A FF

• Cat#No#: Hi-022P
• Product Overview: HiTrap rProtein A FF columns are prepacked with rProtein A Sepharose Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites.
• Characteristic: Quick, convenient preparative purification of monoclonal antibodies.
  Well-established protein A-based resin used in many approved MAb processes.
  Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
  Simple operations with a syringe, pump, or high-performance chromatography system, such as ÄKTA design.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Sample preparation: ample preparation The sample should be adjusted to the composition of the binding buffer. This can be done by either diluting the sample with binding buffer or by buffer exchange using HiTrap Desalting, HiPrep 26/10 Desalting or PD-10 Desalting columns. The sample should be filtered through a 0.45 μm filter or centrifuged immediately before it is applied to the column. Never apply a turbid solution to the column.
• Matrix: cross-linked agarose, 4%, spherical
• Average particle size: ~ 90 μm
• Ligand: Recombinant protein A, (E. coli)
• Dynamic binding capacity: ~ 35 mg human IgG/mL resin
• Recommended flow rate: < 4 ml/min
• Recommended column height: 25 mm
• Chemical stability: Stable to commonly used aqueous buffers, 6 M guanidine hydrochloride, 2% benzyl alcohol, 1 mM NaOH (pH 11), 0.1 M sodium citrate/HCL (pH3), 20% ethanol.
• pH working range: 3 to 10
• CIP stability: 3 to 12
• Temperature stability: 2°C to 40°C
• Storage: 4 to 30°C, 20% Ethanol
• Pack size: 5 × 1 mL
• Dimensions: 7 × 25 mm
• Column volume: 1 mL
• Column i.d.: 7 mm
• Column hardware pressure limit: 5 bar (0.5 MPa)