HiTrap SP HP column

• Cat#No#: Hi-239P
• Product Overview: HiTrap SP HP is a strong cation exchange chromatography column for high-resolution, small-scale protein purification.
• Characteristic: Packed with SP Sepharose High Performance strong cation exchange resin .
  Small (34 µm) bead size delivers high-performance, high-resolution purifications.
  Can be used alone or connected in series.
  Convenient HiTrap format: designed for use with a syringe, peristaltic pump, or chromatography system.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Sample preparation: The sample should be adjusted to the composition of the start buffer by buffer exchange using HiTrap Desalting, HiPrep 26/10 Desalting or PD-10 columns, see Table 5. The sample should be filtered through a 0.45 μm filter or centrifuged immediately before it is applied to the column.
• Metal ion capacity: 0.15 to 0.20 mmol H+/mL resin
• Matrix: Cross-linked agarose, spherical
• Average particle size: ~ 34 μm
• Dynamic binding capacity: ~ 55 mg ribonuclease/mL resin
• Recommended flow rate: 1 mL/min
• Recommended column height: 25 mm
• Chemical stability: Stable to commonly used aqueous buffers, 1.0 M NaOH, 1.0 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol。
• pH working range: 4 to 13
• CIP stability: 3 to 14
• Storage: 4 to 30°C, 0.2 M Sodium Acetate in 20% Ethanol
• Pack size: 5 × 1 mL
• Maximum flow velocity: 4 mL/min
• Dimensions: 7 × 25 mm
• Column volume: 1 mL
• Column i.d.: 7 mm
• Column hardware pressure limit: 0.5 MPa (5 bar,72.5 psi)
• Functional group: – CH2CH2CH2SO3