HiTrap SP Sepharose FF

• Cat#No#: Hi-240P
• Product Overview: HiTrap SP FF is prepacked with SP Sepharose Fast Flow, and is a strong cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.
• Characteristic: Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
  The industry standard for ion exchange chromatography.
  High flow rates and good scale-up potential.
  Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
  Predictable scale-up.
• Applications: For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Sample preparation: The sample should be adjusted to the composition of the start buffer by buffer exchange using HiTrap Desalting, HiPrep 26/10 Desalting or PD-10 columns, see Table 5. The sample should be filtered through a 0.45 μm filter or centrifuged immediately before it is applied to the column.
• Metal ion capacity: 0.18–0.25 mmol H+/ mL resin
• Matrix: Cross-linked agarose, 6%, spherical
• Ionic Exchanger Type: Strong cation
• Particle Size: 45–165 μm
• Average particle size: ~ 90 μm
• Dynamic binding capacity: ~ 70 mg Ribonuclease A/ mL resin
• Recommended column height: 25 mm
• Chemical stability: Stable to commonly used aqueous buffers, 1.0 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 70% ethanol.
• pH working range: 4 to 13
• CIP stability: 3 to 14
• Storage: 4 to 30°C, 0.2 M Sodium Acetate in 20% Ethanol.
• Pack size: 5 × 1 mL
• Dimensions: 7 × 25 mm
• Column volume: 1 mL
• Column i.d.: 7 mm
• Column hardware pressure limit: 5 bar (0.5 MPa)
• Functional group: -CH2CH2CH2SO3 -