HiTrap SP XL

• Cat#No#: Hi-241P
• Product Overview: HiTrap SP XL are strong cation exchangers prepacked with SP Sepharose XL media, optimized for fast, convenient small-scale protein capture using ion exchange (IEX) chromatography.
• Description: Q Sepharose XL and SP Sepharose XL resins have long chains of dextran coupled to a robust, 6% highly cross-linked agarose matrix. The dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities.
• Characteristic: High binding capacities with high flow rates.
  Convenient and affordable for fast, easy separations either alone or connected in series.
  Designed for use with syringe, peristaltic pump, or chromatography system.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Metal ion capacity: 0.18–0.25 mmol H+/ mL resin
• Matrix: Cross-linked agarose, with dextran surface extender, spherical
• Ionic Exchanger Type: Strong cation
• Average particle size: ~ 90 μm
• Dynamic binding capacity: ≥ 160 mg Lysozyme/ mL resin
• Recommended flow rate: 1 mL/min
• Recommended column height: 25 mm
• Chemical stability: Stable to commonly used aqueous buffers, 1.0 M NaOH, 8 M urea, 20% ethanol, 6 M guanidine hydrochloride, 70% ethanol.
• pH working range: 4 to 13
• CIP stability: 3 to 14
• Storage: 4 to 30°C, 0.2 M Sodium Acetate in 20% Ethanol.
• Pack size: 5 × 1 mL
• Maximum flow velocity: 4 mL/min resp. 20 mL/min
• Dimensions: 7 × 25 mm
• Column volume: 1 mL
• Column i.d.: 7 mm
• Column hardware pressure limit: 5 bar (0.5 MPa, 72 psi)
• Functional group: -CH2CH2CH2SO3-